Phosphoproteins

These proteins are subject to a phosphorylation event. Most often, protein phosphorylation affects the conformation of the protein resulting in changes in biological activity, interaction properties and/ or localization.

Parent category	6. Groups of genes
Neighbouring categories	6.1. Essential genes 6.2. Membrane proteins 6.3. GTP-binding proteins 6.4. Phosphoproteins 6.5. Universally conserved proteins 6.6. Poorly characterized/ putative enzymes 6.7. Proteins of unknown function 6.8. Short peptides 6.9. ncRNA 6.10. Pseudogenes
Related categories	protein kinases and phosphatases two-component systems PTS PRD-containing transcription factors phosphorelay response regulator aspartate phosphatases
Overview on the categories (Excel file) All genes and their categories (Excel file)

Phosphoproteins in B. subtilis

Phosphorylation on an Arg residue

• CtsR (R55), phosphorylated by McsB
• AccD
• AhpF
• AlaR
• AlbF (R17)
• Apt
• ArgG
• AroA
• AroF
• AtpH
• BdhA
• BfmBAB (R23)
• ClpC
• ClpP
• ComFA
• ComFC
• ComGA
• ComK
• CsoR (R24)
• DivIVA
• FadB
• FliY
• FlgN
• FrlB
• GanA
• GapA
• GatC
• GgaA
• GltA
• Gmk
• GroEL
• GtaB
• GudB
• Hbs
• HemB
• Hpf
• HrcA
• Icd
• IlvB
• IlvC
• IolE (R216)
• KatA
• LeuB
• LeuC
• LutB
• LutC
• McsA
• Mdh
• MelA (R56)
• MenB
• MntB (R132)
• MtnA

• MtnK
• NadB
• Nin
• OdhA
• OdhB (R300)
• OxdC
• PdxS
• PfkA
• PurA
• RadC
• RecA
• RplK
• RplN (R17)
• RplV (R11)
• RplW
• RpmEB
• RpmGA
• RpoB
• RpoC
• RpsG
• RpsH (R47, R72)
• RpsI
• RpsL
• RpsM
• RpsN
• ScoC
• SfrAA (R226)
• SrfAB
• SsbA
• SsbB
• ThiC
• ThyB
• Tig
• TufA (R384)
• YceE
• YciC
• YdcI
• YdjO
• YerA (R14)
• YfmG
• YheA
• YkyB (R26)
• YlbN
• YloV
• YrvO

• YtxH
• YvrO

• YwpJ

Phosphorylation on an Asp residue: Response regulators of two-component systems

• BceR: phosphorylated by BceS
• CheB: phosphorylated by CheA
• CheV: phosphorylated by CheA
• CheY: phosphorylated by CheA
• CitT: phosphorylated by CitS

• ComA: phosphorylated by ComP
• CssR: phosphorylated by CssS
• DctR: phosphorylated by DctS
• DegU: phosphorylated by DegS
• DesR: phosphorylated by DesK

• GlnL: phosphorylated by GlnK
• LiaR: phosphorylated by LiaS
• LytT: phosphorylated by LytS
• MalR: phosphorylated by MalK
• NatR: phosphorylated by NatK

• PhoP: phosphorylated by PhoR
• ResD: phosphorylated by ResE
• Spo0A: part of the phosphorelay, phosphorylated by Spo0B
• Spo0F: part of the phosphorelay, phosphorylated by KinA, KinB, KinC, KinD, or KinE
• WalR: phosphorylated by WalK

• YbdJ: phosphorylated by YbdK
• YcbL: phosphorylated by YcbM
• YclJ: phosphorylated by YclK
• YdfI: phosphorylated by YdfH
• YesN: phosphorylated by YesM

• YfiK: phosphorylated by YfiJ
• YhcZ: phosphorylated by YhcY
• YkoG: phosphorylated by YkoH
• YrkP: phosphorylated by YrkO
• YvcP: phosphorylated by YvcQ

• YvfU: phosphorylated by YvfT
• YvrHb: phosphorylated by YvrG
• YxdJ: phosphorylated by YxdK
• YxjL: phosphorylated by YxjM

Phosphorylation on a Cys residue

• PRD-type regulator containing a IIB-like domain
  • MtlR: phosphorylated by MtlF

• Enzyme IIB components of the PTS
  • PtsG: glucose permease, EIICBA: phosphorylated by PtsG-IIA domain
  • GamP: glucosamine permease, EIICBA: phosphorylated by GamP-IIA domain
  • MurP: N-acetyl muramic acid-specific phosphotransferase system, EIIBC: likely phosphorylated by PtsG-IIA domain
  • SacP: sucrose permease (high affinity): phosphorylated by PtsG-IIA domain
  • SacX: sucrose permease (low affinity): phosphorylated by PtsG-IIA domain
  • MtlA: mannitol permease: phosphorylated by MtlF
  • GmuB: galactomannan permease: phosphorylated by GmuA
  • TreP: trehalose permease: phosphorylated by PtsG-IIA domain
  • MalP: maltose permease: likely phosphorylated by PtsG-IIA domain
  • FruA: fructose permease: phosphorylated by FruA-IIA domain
  • ManP: mannose permease: phosphorylated by ManP-IIA domain
  • LicB: lichenan permease: phosphorylated by LicA
  • BglP: ß-glucoside permease: phosphorylated by BglP-IIA domain
  • NagP: N-acetylglucosamine permease: phosphorylated by PtsG-IIA domain

Phosphorylation on a His residue

• PTS proteins
  • Enzyme I: autophosphorylated using phosphoenolpyruvate as phosphate donor
  • HPr: phosphorylated by Enzyme I
  • PtsG: glucose permease, EIICBA: phosphorylated by HPr
  • GamP: glucosamine permease, EIICBA: phosphorylated by HPr
  • MtlF: mannitol permease: phosphorylated by HPr
  • GmuA: galactomannan permease: phosphorylated by HPr
  • MalP: maltose permease: phosphorylated by HPr
  • FruA: fructose permease: phosphorylated by HPr
  • ManP: mannose permease: phosphorylated by HPr
  • LevD: fructose permease: phosphorylated by HPr
  • LevE: fructose permease: phosphorylated by LevD
  • LicA: lichenan permease: phosphorylated by HPr
  • BglP: ß-glucoside permease
  • YpqE: unknown EIIA component: phosphorylated by HPr
  • YyzE: truncated PTS IIA protein: might perhaps be phosphorylated by HPr

• Non-PTS proteins controlled by PTS-dependent phosphorylation
  • GlpK: phosphorylated by HPr
  • GlcT: phosphorylated by HPr and by PtsG
  • LicT: phosphorylated by HPr and likely by BglP
  • SacT: phosphorylated by HPr and likely by SacP
  • SacY: phosphorylated by HPr and likely by SacY
  • LevR: phosphorylated by HPr and by LevE
  • LicR: phosphorylated by HPr and likely by LicB
  • ManR: phosphorylated by HPr and likely by ManP
  • MtlR: phosphorylated by HPr and likely by MtlA

• Protein kinases of two-component systems (These kinases are dimeric proteins. Phosphorylation occurs from one subunit to the other (not quite an autophosphorylation).)
  • BceS
  • CheA
  • CitS
  • ComP
  • CssS

• • DctS
  • DegS
  • DesK
  • GlnK
  • KinA

• • KinB
  • KinC
  • KinD
  • KinE
  • LiaS

• • LytS
  • MalK
  • NatK
  • PhoR
  • ResE

• • Spo0B: part of the phosphorelay, phosphorylated by Spo0F
  • WalK
  • YbdK
  • YcbM
  • YclK

• • YdfH
  • YesM
  • YfiJ
  • YhcY
  • YkoH

• • YrkO
  • YvcQ
  • YvfT
  • YvrG
  • YwpD

• • YxdK
  • YxjM

Phosphorylation on a Ser residue

• Crh: phosphorylated by HPrK
• Hpr: phosphorylated by HPrK
• AbrB Soufi et al., 2010
• AhpF Macek et al., 2007
• AroA Macek et al., 2007

• Asd Macek et al., 2007
• CitZ Macek et al., 2007
• CodY Macek et al., 2007
• CotB Nguyen et al., 2016
• CotG Saggese et al., 2014
• DegS Macek et al., 2007
• DhbC Macek et al., 2007

• DhbF Macek et al., 2007
• Eno Macek et al., 2007
• FusA Macek et al., 2007
• GerR Soufi et al., 2010
• GlmM Macek et al., 2007, Eymannet al., 2007

• GroES Soufi et al., 2010
• GyrB Soufi et al., 2010
• IlvC Soufi et al., 2010
• LicB Macek et al., 2007
• ManP Macek et al., 2007

• Mdh Macek et al., 2007
• MetS Soufi et al., 2010
• MtlA Macek et al., 2007
• Ndk Macek et al., 2007
• PdhB Macek et al., 2007

• Pgk Macek et al., 2007
• Pgm Macek et al., 2007
• PnbA Macek et al., 2007
• PupG Macek et al., 2007
• Enzyme I Macek et al., 2007

• Pyk Macek et al., 2007 Eymannet al., 2007
• PyrB Macek et al., 2007
• RecA Soufi et al., 2010
• RocF Soufi et al., 2010
• RpsJ Rosenberg et al., 2015
• RsbRC Macek et al., 2007

• RsbS Macek et al., 2007
• RsbV Macek et al., 2007 Eymannet al., 2007
• SpoIIAA Macek et al., 2007
• SpoVG Macek et al., 2007
• SrfAA Macek et al., 2007

• SrfAB Macek et al., 2007
• SrfAC Macek et al., 2007
• SspA Rosenberg et al., 2015
• SspB Rosenberg et al., 2015
• SucC Macek et al., 2007
• TagE Macek et al., 2007
• ThiC Soufi et al., 2010

• Tpi Macek et al., 2007
• TrmK Macek et al., 2007
• Tsf Macek et al., 2007
• MurQ Macek et al., 2007
• YcnE Macek et al., 2007

• YerA Macek et al., 2007
• YfjC Soufi et al., 2010
• YfkK Macek et al., 2007
• YjbK Soufi et al., 2010
• YpfD Macek et al., 2007

• YpoC Macek et al., 2007
• YqbO Macek et al., 2007
• YtnP Macek et al., 2007
• YukF Soufi et al., 2010
• YurJ Soufi et al., 2010

• YwfI Macek et al., 2007
• YwjH Macek et al., 2007
• YwrJ Soufi et al., 2010

Phosphorylation on a Thr residue

• LtaS
• AroA Eymannet al., 2007
• CotG Saggese et al., 2014
• CpgA Pompeo et al., 2012
• Drm Macek et al., 2007
• Eno Macek et al., 2007
• FbaA Macek et al., 2007
• FusA Macek et al., 2007
• GndA Macek et al., 2007
• GpsB Pompeo et al., 2015
• Hbs Macek et al., 2007
• Ndk Macek et al., 2007
• OppA Macek et al., 2007
• Pgi Macek et al., 2007
• Pgk Macek et al., 2007
• PrkC Macek et al., 2007
• PrkD Pietacket al., 2010
• RsbR Macek et al., 2007 Eymannet al., 2007
• RsbRB Eymannet al., 2007 Macek et al., 2007
• RsbRC Macek et al., 2007
• RsbRD Macek et al., 2007 Eymannet al., 2007
• SodA Macek et al., 2007
• TyrZ Soufi et al., 2010
• YdcC Soufi et al., 2010
• YvcK Foulquier et al., 2014

Phosphorylation on a Tyr residue

• AhpF Macek et al., 2007
• Asd Macek et al., 2007
• EF-G Rosenberg et al., 2015
• EF-Tu Rosenberg et al., 2015
• Eno Macek et al., 2007
• EpsB Elsholz et al., 2007
• EpsE Elsholz et al., 2007
• FlgN Macek et al., 2007
• InfA Macek et al., 2007
• Ldh Macek et al., 2007
• OppA Macek et al., 2007
• PtkA Soufi et al., 2010
• SsbA Mijakovic et al., 2006
• SsbB Mijakovic et al., 2006
• ThiC Soufi et al., 2010
• TuaD Mijakovic et al., 2003

• Ugd Mijakovic et al., 2003, Macek et al., 2007
• YjoA Macek et al., 2007
• YnfE Macek et al., 2007
• YorK Macek et al., 2007

Phosphorylation on either a Ser, Thr or Tyr residue

• AhpC Levine et al., 2007
• AlsD Levine et al., 2007
• AspS Levine et al., 2007
• AtpA Levine et al., 2007
• AtpD Levine et al., 2007

• DnaK Eymannet al., 2007
• DppA Levine et al., 2007
• GapA Macek et al., 2007 Eymannet al., 2007
• GlgP Macek et al., 2007
• GlnA Levine et al., 2007

• GlyA Levine et al., 2007 Eymannet al., 2007
• GroEL Levine et al., 2007 Eymannet al., 2007
• GtaB Levine et al., 2007
• GuaB Eymannet al., 2007

• Icd Levine et al., 2007 Eymannet al., 2007
• MetE Eymannet al., 2007
• PdhD Eymannet al., 2007
• PdxS Levine et al., 2007 Eymannet al., 2007
• PgcA Levine et al., 2007 Eymannet al., 2007

• PstS Levine et al., 2007
• Pta Macek et al., 2007
• RocA Macek et al., 2007
• SdhA Levine et al., 2007 Eymannet al., 2007
• SucD Macek et al., 2007

• ThiG Eymannet al., 2007
• Tig Levine et al., 2007
• Tkt Levine et al., 2007
• TufA Levine et al., 2007 Eymannet al., 2007
• YerB Macek et al., 2007

• YfiY Macek et al., 2007
• YfjR Levine et al., 2007
• YfnI Macek et al., 2007
• YhfK Levine et al., 2007
• YoxD Levine et al., 2007

• YsnF Levine et al., 2007
• YtaG Levine et al., 2007
• YtxJ Macek et al., 2007
• SufC Levine et al., 2007
• WapI Macek et al., 2007

• Zur Levine et al., 2007