purA

purA
168

adenylosuccinate synthetase

Locus
BSU_40420
Molecular weight
47.51 kDa
Isoelectric point
5.46
Protein length
430 aaSequenceBlast
Gene length
1293 bpSequenceBlast
Function
purine biosynthesis
Product
adenylosuccinate synthetase
Essential
no
E.C.
6.3.4.4
Synonyms
purA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG0104 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
4,155,433 4,156,725
The protein
Catalyzed reaction/ biological activity
GTP + IMP + L-aspartate --> GDP + 2 H+ + N6-(1,2-dicarboxyethyl)-AMP + phosphate (according to UniProt)
Protein family
adenylosuccinate synthetase family (single member, according to UniProt)
Structure
4M0G (PDB) (from B. anthracis, 80.1% identity)
P29726 (AlphaFold)
Modification
phosphorylated on Arg-97 PubMed
Localization
cytoplasm (according to Swiss-Prot)
Additional information
belongs to the 100 most abundant proteins PubMed
Expression and Regulation
Operons
Genes
purA
Description
PubMed
Regulation
expression activated by glucose (4.9 fold) (CcpA) PubMed
Regulatory mechanism
PurR: repression, PubMed, in purR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

purA

2025-03-19 06:54:43

ghost

79

7be4579d3311ce9ff90d905b45d6cc86e7ad6cfe

1532A476F292DF14E49CE718A58ED75916B270E2

Genes
yycE-purA
Description
PubMed
Open in new tab

yycE→purA

2025-03-16 16:53:00

Jstuelk

92

4a912c55b3406aa5ba6f1c5c8e433c424892e12d

AFBAF653829816EA925A4E727CC7A8D893DA8987

Biological materials
Mutant
BKE40420 (purA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTCCGTGCACCTCCGTT, downstream forward: _UP4_TAAATAGAATATGTCTGCAA
BKK40420 (purA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTCCGTGCACCTCCGTT, downstream forward: _UP4_TAAATAGAATATGTCTGCAA
References
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AB4B8B7325A1BF444D841EF1BBBCEC2D506AE600

Page visits: 5668

Time of last update: 2025-04-02 13:57:26

Author of last update: Jstuelk