mtnA

mtnA
168

5-methylthioribose-1-phosphate isomerase

Locus
BSU_13550
Molecular weight
38.70 kDa
Isoelectric point
5
Protein length
353 aaSequenceBlast
Gene length
1062 bpSequenceBlast
Function
methionine salvage
Product
5-methylthioribose-1-phosphate isomerase
Essential
no
E.C.
5.3.1.23
Synonyms
mtnA, ykrS, mtnS
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0182 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,422,172 1,423,233
The protein
Catalyzed reaction/ biological activity
S-methyl-5-thio--D-ribose 1-phosphate --> 5-methylsulfanyl-D-ribulose 1-phosphate (according to UniProt)
Protein family
eIF-2B alpha/beta/delta subunits family (single member, according to UniProt)
Structure
2YVK (PDB)
O31662 (AlphaFold)
Modification
phosphorylated on Arg-80 and Arg-298 PubMed
Additional information
subject to Clp-dependent proteolysis upon glucose starvation PubMed
belongs to the 100 most abundant proteins PubMed
Expression and Regulation
Operons
Genes
mtnK-mtnA
Description
PubMed
Regulation
induced by methionine starvation (S-box) PubMed
NusG-dependent transcription pausing precedes the completion of the SAM-binding aptamer domain of the S-box riboswitch PubMed
the S-box RNA is degraded by RNase Y PubMed
expression is reduced in motile cells as compared to non-motile cells PubMed
Regulatory mechanism
S-box: RNA switch, , the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed, in S-box
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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mtnK→mtnA

2025-04-02 10:11:03

Jstuelk

100

5E437FB23A4F009B0E9A667E3F04D466554C0F58

2EB65B4B0A58D80719B6A9288A8B2BEF4BC3FFF2

Biological materials
Mutant
MGNA-B320 (ykrS::erm), available at the NBRP B. subtilis, Japan
BKE13550 (mtnA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AGCAAATGAATGGGTCATGA, downstream forward: _UP4_TAAAAAACCGCTGGACTTTG
BKK13550 (mtnA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AGCAAATGAATGGGTCATGA, downstream forward: _UP4_TAAAAAACCGCTGGACTTTG
References
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32DD5C61F0F8147DEACBCBBE1283B4E2C88E96E9

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Time of last update: 2025-04-02 10:32:50

Author of last update: Jstuelk