hpf

hpf
168

general stress protein, required for ribosome dimerization in the stationary phase, protects essential ribosomal proteins (S2 and S3)

Locus
BSU_35310
Molecular weight
21.83 kDa
Isoelectric point
5.18
Protein length
189 aaSequenceBlast
Gene length
570 bpSequenceBlast
Function
protection of essential ribosomal proteins (S2 and S3)
Product
ribosome hibernation promoting factor
Essential
no
Synonyms
hpf, yviI, yvyD
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1544 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,631,003  3,631,572
Phenotypes of a mutant
the mutant is cold-sensitive
delayed recovery from stationary phase and delayed germination PubMed
The protein
Catalyzed reaction/ biological activity
Hpf binds the 30S subunits of hibernating ribosomes, dimerization of Hpf results in 100S ribosome dimer formation PubMed
Protein family
HPF/YfiA ribosome-associated protein family (single member, according to UniProt)
Structure
5NJT (PDB) (N-terminal domain (aa 1 ... 104) of Hpf as part of the 100S ribosome) PubMed
8UU4 (PDB) (complex with the ribosome, from Listeria monocytogenes, 60.3% identity) PubMed
P28368 (AlphaFold)
Modification
phosphorylated on Arg-6 PubMed
Localization
cytoplasm (according to UniProt)
Additional information
Hpf is degraded in the forespore using MdfA as the adaptor protein PubMed
Expression and Regulation
Operons
Genes
yvyC-fliD-fliS-fliT-smiA-S1350-hpf
Description
PubMed
Sigma factors
SigD: sigma factor, PubMed, in sigD regulon
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yvyC->hpf

2025-03-17 22:24:00

Jstuelk

110

e3ca620c52dfbad7e213327c6d00bc708a17de60

8A78945BE9E5D0307404BC35D532282B9C61997C

Genes
hpf
Description
PubMed
Regulation
expressed in the stationary phase PubMed
Regulatory mechanism
PhoP: activation, PubMed, in phoP regulon
Sigma factors
SigB: sigma factor, PubMed, in sigB regulon
SigH: sigma factor, PubMed, in sigH regulon
Open in new tab

hpf

2025-03-17 19:58:21

ghost

64

ff2be4c48a1589a5efe0935c849474ba3178f026

981F17CD0A259AA7DDA858A2058F3F74F777952D

Biological materials
Mutant
BKE35310 (hpf::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCAAAGAACGCCTCCCTT,  downstream forward: _UP4_TAATGAAGAGAAGCCTTCCG
BKK35310 (hpf::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCAAAGAACGCCTCCCTT,  downstream forward: _UP4_TAATGAAGAGAAGCCTTCCG
GP2598 (hpf::tet comIQ12L) (in DK1042) available in Jörg Stülke's lab
References
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Time of last update: 2025-04-02 01:50:39

Author of last update: Jstuelk