srfAC

srfAC
168

surfactin synthetase / competence

Locus
BSU_03510
Molecular weight
143.59 kDa
Isoelectric point
4.97
Protein length
1275 aaSequenceBlast
Gene length
3828 bpSequenceBlast
Function
antibiotic synthesis
Product
surfactin synthetase / competence
Essential
no
Synonyms
srfAC, comL
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG3319 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
398,532  402,359
The protein
Protein family
ATP-dependent AMP-binding enzyme family (according to UniProt)
Domains
Carrier domain (aa 968-1043) (according to UniProt)
Structure
2VSQ (PDB) PubMed
Q08787 (AlphaFold)
Modification
phosphorylation on Ser-1003 PubMed
Localization
cell membrane PubMed
Additional information
the protein can be degraded by ClpC-ClpP PubMed
Expression and Regulation
Operons
Genes
srfAA-srfAB-comS-srfAC-srfAD
Description
PubMed
Regulation
expressed at high cell density (ComA) PubMed
heterogeneous expression, percentage of expressing cells increases during colonization of plant roots PubMed
Regulatory mechanism
PhoP: activation, PubMed, in phoP regulon
CodY: repression, PubMed, in codY regulon
ComA: activation, PubMed, in comA regulon
PerR: activation, PubMed, in perR regulon
Spx: repression, PubMed, in spx regulon
Abh: repression, PubMed, in abh regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Additional information
A ncRNA is predicted between 'HxlR' and 'SrfAA' PubMed
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srfAA→srfAD

2025-03-31 22:54:29

Jstuelk

157

620D29280C2CC5BBF61DFDE1E763667777B28513

4F5B391B1D8CC1181EDE8EB2DAC874A9B90A39CA

Biological materials
Mutant
BKE03510 (srfAC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATGTTTCGCTCCCGCCT,  downstream forward: _UP4_TAAATGAAGTGATGAAAGGA
BKK03510 (srfAC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATGTTTCGCTCCCGCCT,  downstream forward: _UP4_TAAATGAAGTGATGAAAGGA
References
Reviews
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Research papers
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1D5F227860A321506A1AB4BAE63CAD3A66E43BFC

Page visits: 5426

Time of last update: 2025-04-02 11:08:34

Author of last update: Jstuelk