kinE

kinE
168

two-component sensor kinase, phosphorylates Spo0F, part of the phosphorelay

Locus
BSU_13530
Molecular weight
85.32 kDa
Isoelectric point
5.49
Protein length
738 aaSequenceBlast
Gene length
2217 bpSequenceBlast
Function
initiation of sporulation
Product
two-component sensor kinase
Essential
no
Synonyms
kinE, ykrQ
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG5809 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,419,213  1,421,429
The protein
Catalyzed reaction/ biological activity
autophosphorylation, phosphorylation of Spo0F
ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)
Domains
two transmembrane segments
4 PAS domains (aa 29-99, aa 150-220, aa 271-342, aa 391-462) (according to UniProt)
Histidine kinase domain (aa 523-729) (according to UniProt)
Structure
3D36 (PDB) (Geobacillus stearothermophilus KinB in complex with the inhibitor Sda, corresponds to aa 510 ... 727, 43% identity) PubMed
O31661 (AlphaFold)
Modification
autophosphorylation on a His residue
Paralogous protein(s)
CssS, YclK, YvrG, YkoH
Expression and Regulation
Operons
Genes
kinE-ogt
Description
PubMed
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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kinE→ogt

2025-03-15 07:58:25

ghost

117

63e752d8925a0480f853181e8e69a5b2e969362b

4B7DD3F359520D4DAAB003D0DC6AF3C2D719EB36

Biological materials
Mutant
MGNA-B319 (ykrQ::erm), available at the NBRP B. subtilis, Japan
BKE13530 (kinE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCATGTACACTCCTTCT,  downstream forward: _UP4_GAATGAACTACTATACGACA
BKK13530 (kinE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCATGTACACTCCTTCT,  downstream forward: _UP4_GAATGAACTACTATACGACA
References
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Time of last update: 2025-03-31 16:52:06

Author of last update: Jstuelk