Phosphoproteins

These proteins are subject to a phosphorylation event. Most often, protein phosphorylation affects the conformation of the protein resulting in changes in biological activity and/ or localization.

Phosphoproteins in B. subtilis

Phosphorylation on an Arg residue

• CtsR, phosphorylated by McsB

Phosphorylation on an Asp residue: Response regulators of two-component systems

• ComA: phosphorylated by ComP
• DegU: phosphorylated by DegS
• DesR: phosphorylated by DesK
• LiaR: phosphorylated by LiaS
• YdfI: phosphorylated by YdfH
• YfiK: phosphorylated by YfiJ
• YhcZ: phosphorylated by YhcY
• YvfU: phosphorylated by YvfT
• YxjL: phosphorylated by YxjM
• BceR: phosphorylated by BceS
• CssR: phosphorylated by CssS
• NatR: phosphorylated by NatK
• PhoP: phosphorylated by PhoR
• ResD: phosphorylated by ResE
• WalR: phosphorylated by WalK
• YbdJ: phosphorylated by YbdK
• YcbL: phosphorylated by YcbM
• YclJ: phosphorylated by YclK
• YkoG: phosphorylated by YkoH
• YrkP: phosphorylated by YrkO
• YvcP: phosphorylated by YvcQ
• YvrHb: phosphorylated by YvrG
• YxdJ: phosphorylated by YxdK
• CheY: phosphorylated by CheA
• CitT: phosphorylated by CitS
• DctR: phosphorylated by DctS
• GlnL: phosphorylated by GlnK
• MalR: phosphorylated by MalK
• LytT: phosphorylated by LytS
• YesN: phosphorylated by YesM
• Spo0F: part of the phosphorelay, phosphorylated by KinA, KinB, KinC, KinD, or KinE
• Spo0A: part of the phosphorelay, phosphorylated by Spo0B

Phosphorylation on a Cys residue: Enzyme IIB components of the PTS

• PtsG: glucose permease, EIICBA: phosphorylated by PtsG-IIA domain
• GamP: glucosamine permease, EIICBA: phosphorylated by GamP-IIA domain
• MurP: N-acetyl muramic acid-specific phosphotransferase system, EIIBC: likely phosphorylated by PtsG-IIA domain
• SacP: sucrose permease (high affinity): phosphorylated by PtsG-IIA domain
• SacX: sucrose permease (low affinity): phosphorylated by PtsG-IIA domain
• MtlA: mannitol permease: phosphorylated by MtlF
• GmuB: galactomannan permease: phosphorylated by GmuA
• TreP: trehalose permease: phosphorylated by PtsG-IIA domain
• MalP: maltose permease: likely phosphorylated by PtsG-IIA domain
• FruA: fructose permease: phosphorylated by FruA-IIA domain
• ManP: mannose permease: phosphorylated by ManP-IIA domain
• LicB: lichenan permease: phosphorylated by LicA
• BglP: ß-glucoside permease: phosphorylated by BglP-IIA domain
• NagP: N-acetylglucosamine permease: phosphorylated by PtsG-IIA domain

Phosphorylation on a His residue

• PTS proteins
  • Enzyme I: autophosphorylated using phosphoenolpyruvate as phosphate donor
  • HPr: phosphorylated by Enzyme I
  • PtsG: glucose permease, EIICBA: phosphorylated by HPr
  • GamP: glucosamine permease, EIICBA: phosphorylated by HPr
  • MtlF: mannitol permease: phosphorylated by HPr
  • GmuA: galactomannan permease: phosphorylated by HPr
  • MalP: maltose permease: phosphorylated by HPr
  • FruA: fructose permease: phosphorylated by HPr
  • ManP: mannose permease: phosphorylated by HPr
  • LevD: fructose permease: phosphorylated by HPr
  • LevE: fructose permease: phosphorylated by LevD
  • LicA: lichenan permease: phosphorylated by HPr
  • BglP: ß-glucoside permease
  • YpqE: unknown EIIA component: phosphorylated by HPr
  • YyzE: truncated PTS IIA protein: might perhaps be phosphorylated by HPr

• Non-PTS proteins controlled by PTS-dependent phosphorylation
  • GlpK: phosphorylated by HPr
  • GlcT: phosphorylated by HPr and by PtsG
  • LicT: phosphorylated by HPr and likely by BglP
  • SacT: phosphorylated by HPr and likely by SacP
  • SacY: phosphorylated by HPr and likely by SacY
  • LevR: phosphorylated by HPr and by LevE
  • LicR: phosphorylated by HPr and likely by LicB
  • ManR: phosphorylated by HPr and likely by ManP
  • MtlR: phosphorylated by HPr and likely by MtlA

• Protein kinases of two-component systems (These kinases are dimeric proteins. Phosphorylation occurs from one subunit to the other (not quite an autophosphorylation).)
  • ComP
  • DegS
  • DesK
  • LiaS
  • YdfH
  • YfiJ
  • YhcY
  • YvfT
  • YxjM
  • BceS
  • CssS
  • NatK
  • PhoR
  • ResE
  • WalK
  • YbdK
  • YcbM
  • YclK
  • YkoH
  • YrkO
  • YvcQ
  • YvrG
  • YxdK
  • CheA
  • CitS
  • DctS
  • GlnK
  • MalK
  • LytS
  • YesM
  • YwpD
  • KinA
  • KinB
  • KinC
  • KinD
  • KinE
  • Spo0B: part of the phosphorelay, phosphorylated by Spo0F

Phosphorylation on a Ser residue

• Crh: phosphorylated by HPrK
• Hpr: phosphorylated by HPrK
• AhpF Macek et al., 2007
• AroA Macek et al., 2007
• Asd Macek et al., 2007
• CitZ Macek et al., 2007
• CodY Macek et al., 2007
• DegS Macek et al., 2007
• DhbC Macek et al., 2007
• DhbF Macek et al., 2007
• Eno Macek et al., 2007
• FusA Macek et al., 2007
• GlmM Macek et al., 2007 Eymannet al., 2007
• LicB Macek et al., 2007
• ManP Macek et al., 2007
• Mdh Macek et al., 2007
• MtlA Macek et al., 2007
• Ndk Macek et al., 2007
• PdhB Macek et al., 2007
• Pgk Macek et al., 2007
• Pgm Macek et al., 2007
• PnbA Macek et al., 2007
• Pnp Macek et al., 2007
• Enzyme I Macek et al., 2007
• Pyk Macek et al., 2007 Eymannet al., 2007
• PyrB Macek et al., 2007
• RsbRC Macek et al., 2007
• RsbS Macek et al., 2007
• RsbV Macek et al., 2007 Eymannet al., 2007
• SpoIIAA Macek et al., 2007
• SpoVG Macek et al., 2007
• SrfAA Macek et al., 2007
• SrfAB Macek et al., 2007
• SrfAC Macek et al., 2007
• SucC Macek et al., 2007
• TagE Macek et al., 2007
• Tpi Macek et al., 2007
• TrmK Macek et al., 2007
• Tsf Macek et al., 2007
• YbbI Macek et al., 2007
• YcnE Macek et al., 2007
• YerA Macek et al., 2007
• YfkK Macek et al., 2007
• YpfD Macek et al., 2007
• YpoC Macek et al., 2007
• YqbO Macek et al., 2007
• YtnP Macek et al., 2007
• YwfI Macek et al., 2007
• YwjH Macek et al., 2007

Phosphorylation on a Thr residue

• LtaS
• AroA Eymannet al., 2007
• Drm Macek et al., 2007
• Eno Macek et al., 2007
• FbaA Macek et al., 2007
• FusA Macek et al., 2007
• GndA Macek et al., 2007
• Hbs Macek et al., 2007
• IspU Macek et al., 2007
• Ndk Macek et al., 2007
• OppA Macek et al., 2007
• Pgi Macek et al., 2007
• Pgk Macek et al., 2007
• PrkC Macek et al., 2007
• PrkD Pietacket al., 2010
• RsbR Macek et al., 2007 Eymannet al., 2007
• RsbRB Eymannet al., 2007
• RsbRC Macek et al., 2007
• RsbRD Macek et al., 2007 Eymannet al., 2007
• SodA Macek et al., 2007

Phosphorylation on a Tyr residue

• AhpF Macek et al., 2007
• Asd Macek et al., 2007
• Eno Macek et al., 2007
• InfA Macek et al., 2007
• Ldh Macek et al., 2007
• OppA Macek et al., 2007
• YjoA Macek et al., 2007
• YnfE Macek et al., 2007
• YorK Macek et al., 2007
• YvyG Macek et al., 2007
• YwqF Macek et al., 2007

Phosphorylation on either a Ser, Thr or Tyr residue

• AhpC Levine et al., 2007
• AlsD Levine et al., 2007
• AspS Levine et al., 2007
• AtpA Levine et al., 2007
• AtpD Levine et al., 2007
• DnaK Eymannet al., 2007
• DppA Levine et al., 2007
• GapA Macek et al., 2007 Eymannet al., 2007
• GlgP Macek et al., 2007
• GlnA Levine et al., 2007
• GlyA Levine et al., 2007 Eymannet al., 2007
• GpsB Macek et al., 2007
• GroEL Levine et al., 2007 Eymannet al., 2007
• GtaB Levine et al., 2007
• GuaB Eymannet al., 2007
• Icd Levine et al., 2007 Eymannet al., 2007
• MetE Eymannet al., 2007
• PdxS Levine et al., 2007 Eymannet al., 2007
• PgcA Levine et al., 2007 Eymannet al., 2007
• PstS Levine et al., 2007
• Pta Macek et al., 2007
• RocA Macek et al., 2007
• SdhA Levine et al., 2007 Eymannet al., 2007
• SucD Macek et al., 2007
• ThiG Eymannet al., 2007
• Tig Levine et al., 2007
• Tkt Levine et al., 2007
• TufA Levine et al., 2007 Eymannet al., 2007
• YerB Macek et al., 2007
• YfiY Macek et al., 2007
• YfjR Levine et al., 2007
• YfnI Macek et al., 2007
• YhfK Levine et al., 2007
• YoxD Levine et al., 2007
• YsnF Levine et al., 2007
• YtaG Levine et al., 2007
• YtxJ Macek et al., 2007
• YurY Levine et al., 2007
• YxxG Macek et al., 2007
• Zur Levine et al., 2007

Related Lists

• two-component systems
• PTS
• PRD-containing transcription factors
• phosphorelay
• response regulator aspartate phosphatases