Bcd
- Description: valine dehydrogenase, isoleucine dehydrogenase, L-leucine dehydrogenase
Gene name | bcd |
Synonyms | bkd, yqiT |
Essential | no |
Product | valine dehydrogenase, isoleucine dehydrogenase, L-leucine dehydrogenase |
Function | utilization of branched-chain keto acids |
Gene expression levels in SubtiExpress: bcd | |
Metabolic function and regulation of this protein in SubtiPathways: bcd | |
MW, pI | 39 kDa, 4.942 |
Gene length, protein length | 1092 bp, 364 aa |
Immediate neighbours | buk, ptb |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU24080
Phenotypes of a mutant
Database entries
- BsubCyc: BSU24080
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: L-leucine + H2O + NAD+ = 4-methyl-2-oxopentanoate + NH3 + NADH (according to Swiss-Prot)
- Protein family: Glu/Leu/Phe/Val dehydrogenases family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
Database entries
- BsubCyc: BSU24080
- UniProt: P54531
- KEGG entry: [3]
- E.C. number: 1.4.1.9
Additional information
Expression and regulation
- Additional information:
- number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 3408 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 2690 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 11556 PubMed
Biological materials
- Mutant:
- a bcd mutant and a bcd ybgE ywaA triple mutant are available in Linc Sonenshein's lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Original publications