bkdAA
168

2-oxoisovalerate dehydrogenase (E1 alpha subunit)

Locus

BSU_24050

Molecular weight

36.18 kDa

Isoelectric point

4.78

Protein length

330 aaSequenceBlast

Gene length

993 bpSequenceBlast

Function

utilization of branched-chain keto acids

Product

2-oxoisovalerate dehydrogenase (E1 alpha subunit)

Essential

E.C.

1.2.4.4

Synonyms

bkdAA, bfmBAA, bfmB1a, bkd

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1071 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene

Coordinates

2,499,090 2,500,082

Phenotypes of a mutant

the mutant tends to acquire suppressor mutations that result in improved growth PubMed

a strain lacking the ptb-bcd-buk-lpdV-bkdAA-bkdAB-bkdB operon and the des gene exhibits strongly reduced membrane fluidity and eventual growth arrest PubMed

The protein

Catalyzed reaction/ biological activity

3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase]-(R)-N⁶-lipoyl-L-lysine + H⁺ --> [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase]-(R)-N⁶-(S⁸-2-methylpropanoyldihydrolipoyl)-L-lysine + CO₂ (according to UniProt)

Protein family

BCKDHA family (single member, according to UniProt)

Cofactors

TPP PubMed

Structure

1UMB (PDB) (from Thermus thermophilus, 42% identity, 57% similarity) PubMed

P37940 (AlphaFold)

Paralogous protein(s)

AcoA, PdhA

Localization

Membrane-proximal (Spotty) PubMed

Expression and Regulation