ilvE

ilvE
168

branched-chain amino acid aminotransferase

Locus
BSU_02390
Molecular weight
39.52 kDa
Isoelectric point
4.95
Protein length
356 aaSequenceBlast
Gene length
1071 bpSequenceBlast
Function
biosynthesis of branched-chain amino acids
Product
branched-chain amino acid aminotransferase
Essential
no
E.C.
2.6.1.42
Synonyms
ybgE
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0115 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
259,016  260,086
The protein
Catalyzed reaction/ biological activity
2-oxoglutarate + L-leucine --> 4-methyl-2-oxopentanoate + L-glutamate (according to UniProt)
2-oxoglutarate + L-isoleucine --> (S)-3-methyl-2-oxopentanoate + L-glutamate (according to UniProt)
2-oxoglutarate + L-valine --> 3-methyl-2-oxobutanoate + L-glutamate (according to UniProt)
Protein family
Class-IV pyridoxal-phosphate-dependent aminotransferase family (according to UniProt)
Cofactors
PLP
Structure
3HT5 (PDB) (from Mycobacterium tuberculosis, 48% identity) PubMed
O31461 (AlphaFold)
Paralogous protein(s)
IlvK
Expression and Regulation
Operons
Genes
ilvE
Description
PubMed
Regulation
repressed by casamino acids PubMed
Regulatory mechanism
CodY: repression, PubMed, in codY regulon
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ilvE

2025-03-19 14:52:21

ghost

79

05683f8a084501862bd43ed443da129c038c724b

C5CC412F9DE1EA3AC16757F8A7844CCD0896F252

Biological materials
Mutant
MGNA-B939 (ybgE::erm), available at the NBRP B. subtilis, Japan
a ybgE mutant and a ''bcd ilvE ilvK'' triple mutant are available in Linc Sonenshein's lab
BKE02390 (ilvE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATCTAATCAGCACACCTT,  downstream forward: _UP4_TGAAAATCGAAAAAGAACCT
BKK02390 (ilvE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATCTAATCAGCACACCTT,  downstream forward: _UP4_TGAAAATCGAAAAAGAACCT
LacZ fusion
pGP248 (in pAC5), available in Jörg Stülke's lab
References
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2A20EF701B9F78FC21A33D6BD8ED862323BA59A8

Page visits: 6146

Time of last update: 2025-04-01 18:28:41

Author of last update: Robert.warneke