ptb

ptb
168

phosphate butyryltransferase

Locus
BSU_24090
Molecular weight
31.62 kDa
Isoelectric point
7.94
Protein length
299 aaSequenceBlast
Gene length
900 bpSequenceBlast
Function
utilization of branched-chain keto acids
Product
phosphate butyryltransferase
Essential
no
E.C.
2.3.1.19
Synonyms
ptb, yqiS, bkd
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0280 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,503,765  2,504,664
The protein
Catalyzed reaction/ biological activity
butanoyl-CoA + phosphate --> butanoyl phosphate + CoA (according to UniProt)
Protein family
phosphate acetyltransferase and butyryltransferase family (with Pta, according to UniProt)
Structure
1YCO (PDB) (from Enterococcus faecalis v583, 36% identity, 56% similarity)
P54530 (AlphaFold)
Expression and Regulation
Operons
Genes
ptb-bcd-buk-lpdV-bkdAA-bkdAB-bkdB
Description
PubMed
Regulation
induced in the presence of isoleucine or valine (BkdR) PubMed
Regulatory mechanism
BkdR: activation, PubMed, in bkdR regulon
CodY: repression, PubMed, in codY regulon
Sigma factors
SigL: sigma factor, PubMed, in sigL regulon
Open in new tab

ptb→bkdB

2025-03-16 17:10:53

ghost

100

7b8921dc3f8fdb775067a1f92187895088ac1126

0BF3F61A9229E387986A64E3D2F9F647D24EA48A

Biological materials
Mutant
MGNA-C417 (yqiS::erm), available at the NBRP B. subtilis, Japan
BKE24090 (ptb::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTGTTACCACCTTTCTT,  downstream forward: _UP4_TACACACATTAGGAGGAACG
BKK24090 (ptb::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTGTTACCACCTTTCTT,  downstream forward: _UP4_TACACACATTAGGAGGAACG
References
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1CFF7202AC6078B6BAD1253F77FD4FE227C046DB

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Time of last update: 2025-04-03 01:48:36

Author of last update: Melvin.boenninger