SsbA
Revision as of 14:15, 17 April 2014 by 134.76.70.252 (talk)
- Description: single-strand DNA-binding protein, part of the replisome
Gene name | ssbA |
Synonyms | ssb |
Essential | yes PubMed |
Product | single-strand DNA-binding protein |
Function | DNA replication, DNA repair/ recombination |
Gene expression levels in SubtiExpress: ssbA | |
Interactions involving this protein in SubtInteract: SsbA | |
MW, pI | 18 kDa, 4.822 |
Gene length, protein length | 516 bp, 172 aa |
Immediate neighbours | rpsR, rpsF |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
DNA replication, DNA repair/ recombination, essential genes, phosphoproteins
This gene is a member of the following regulons
ComK regulon, stringent response
The gene
Basic information
- Locus tag: BSU40900
Phenotypes of a mutant
essential PubMed
Database entries
- BsubCyc: BSU40900
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s): SsbB
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity: Phosphorylation of Ssb increases binding to single-stranded DNA in vitro almost 200-fold PubMed
- Interactions:
- forms tetramers PubMed
- part of the replisome: PolC-HolA-HolB-DnaX-DnaN-DnaG-DnaC-DnaI-DnaD-SsbA-DnaE-PriA-DnaB PubMed
- SsbA-SbcC PubMed
- SsbA-RecG PubMed
- SsbA-YrvE PubMed
- SsbA-YrvN PubMed
- SsbA (bound to ssDNA) -RecO, this helps to dislodge SsbA and SsbB from ssDNA PubMed
- SsbA-YqiB PubMed
- SsbA-Ung PubMed
- SsbA-YpbB PubMed
- SsbA-RecD2 PubMed
- SsbA-DnaN PubMed
- SsbA (bound to ssDNA) -RecO, this helps to dislodge SsbA and SsbB from ssDNA PubMed
- SsbA-YocI according to PubMed
- SsbA-RecQ according to PubMed
Database entries
- BsubCyc: BSU40900
- UniProt: P37455
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Additional information:
- the mRNA contains a structured motif in the 5' UTR upstream of the rpsF ORF that is bound by RpsF-RpsR heterodimers and may be implicated in autogenous regulation PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium): 279 PubMed
- number of protein molecules per cell (complex medium with amino acids, without glucose): 2199 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 263 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 147 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 3701 PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Dusica Vujaklija, Boris Macek
Detecting posttranslational modifications of bacterial SSB proteins.
Methods Mol Biol: 2012, 922;205-18
[PubMed:22976189]
[WorldCat.org]
[DOI]
(I p)
Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559]
[WorldCat.org]
[DOI]
(I p)
Original publications