rarA

rarA
168

DNA-dependent ATPase, recombination factor, involved in processing recombination intermediates at replication forks

Locus
BSU_27530
Molecular weight
46.35 kDa
Isoelectric point
6.8
Protein length
421 aaSequenceBlast
Gene length
1266 bpSequenceBlast
Function
control of the access of the replication machinery to a collapsed replication fork
Product
RecA accessory protein, DNA-dependent ATPase
Essential
no
Synonyms
rarA, yrvN
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2256 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,812,336  2,813,601
Phenotypes of a mutant
sensitive to H2O2 PubMed
reduced viability of rarA recA or rarA recO double mutants PubMed
The protein
Catalyzed reaction/ biological activity
inhibits PriA-dependent initiation of DNA replication PubMed
contributes to error-prone DNA repair, links re-initiation of DNA replication with repair-by-recombination by controlling the access of the replication machinery to a collapsed replication fork PubMed
contributes to the assembly of RecA nucleoprotein filaments onto single-stranded DNA PubMed
Protein family
AAA ATPase family (according to UniProt)
Structure
3PVS (PDB) (from E. coli, 39% identity) PubMed
O34528 (AlphaFold)
Effectors of protein activity
ATPase is stimulated by ssDNAdsDNA junctions or dsDNA ends PubMed
ssDNA-dependent ATPase is stimulated by SsbA bound to ssDNA PubMed
Expression and Regulation
Operons
Genes
rarA
Description
PubMed
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rarA

2025-02-01 16:52:25

Jstuelk

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9a8f06e91ad8a0bcddb3842188b5aa73060077c3

BEF8F3B7D60BB1C293D8E09BA99032BF88A93DF6

Biological materials
Mutant
MGNA-A827 (yrvN::erm), available at the NBRP B. subtilis, Japan
BKE27530 (rarA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTATCATCTCTCCTTAC,  downstream forward: _UP4_TAAAACAAAAAAACAGCCTT
BKK27530 (rarA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTATCATCTCTCCTTAC,  downstream forward: _UP4_TAAAACAAAAAAACAGCCTT
References
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Time of last update: 2025-04-03 19:24:01

Author of last update: Jstuelk