CodV

• Description: site-specific integrase/recombinase
  
  

Gene name	codV
Synonyms
Essential	no
Product	site-specific integrase/recombinase
Function	chromosome partitioning
Gene expression levels in SubtiExpress: codV
MW, pI	35 kDa, 9.31
Gene length, protein length	912 bp, 304 aa
Immediate neighbours	trmFO, clpQ
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA condensation/ segregation

This gene is a member of the following regulons

CodY regulon

The gene

Basic information

• Locus tag: BSU16140

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: resolution of chromosome dimers by site specific recombination at the dif site (located close to the terminus region on the chromosome)

• Protein family: XerC subfamily (according to Swiss-Prot)

• Paralogous protein(s): RipX

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)
  • assembles on the chromosome at the dif site throughout the cell cycle PubMed

Database entries

• Structure:

• UniProt: P39776

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: codV-clpQ-clpY-codY PubMed

• Expression browser: codV PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A794 ( codV::kan), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Christine Kaimer, Katrin Schenk, Peter L Graumann
Two DNA translocases synergistically affect chromosome dimer resolution in Bacillus subtilis.
J Bacteriol: 2011, 193(6);1334-40
[PubMed:21239579] [WorldCat.org] [DOI] (I p)

Alexandra E Bloor, Rocky M Cranenburgh
An efficient method of selectable marker gene excision by Xer recombination for gene replacement in bacterial chromosomes.
Appl Environ Microbiol: 2006, 72(4);2520-5
[PubMed:16597952] [WorldCat.org] [DOI] (P p)

M Ratnayake-Lecamwasam, P Serror, K W Wong, A L Sonenshein
Bacillus subtilis CodY represses early-stationary-phase genes by sensing GTP levels.
Genes Dev: 2001, 15(9);1093-103
[PubMed:11331605] [WorldCat.org] [DOI] (P p)

S A Sciochetti, P J Piggot, G W Blakely
Identification and characterization of the dif Site from Bacillus subtilis.
J Bacteriol: 2001, 183(3);1058-68
[PubMed:11208805] [WorldCat.org] [DOI] (P p)

S A Sciochetti, P J Piggot, D J Sherratt, G Blakely
The ripX locus of Bacillus subtilis encodes a site-specific recombinase involved in proper chromosome partitioning.
J Bacteriol: 1999, 181(19);6053-62
[PubMed:10498718] [WorldCat.org] [DOI] (P p)

F J Slack, P Serror, E Joyce, A L Sonenshein
A gene required for nutritional repression of the Bacillus subtilis dipeptide permease operon.
Mol Microbiol: 1995, 15(4);689-702
[PubMed:7783641] [WorldCat.org] [DOI] (P p)