Phosphoproteins

These proteins are subject to a phosphorylation event. Most often, protein phosphorylation affects the conformation of the protein resulting in changes in biological activity and/ or localization.

Phosphoproteins in B. subtilis

Phosphorylation on an Arg residue

• CtsR, phosphorylated by McsB

Phosphorylation on a His residue

• PTS proteins
  • Enzyme I: autophosphorylated using phosphoenolpyruvate as phosphate donor
  • HPr: phosphorylated by Enzyme I
  • PtsG: glucose permease, EIICBA: phosphorylated by HPr
  • GamP: glucosamine permease, EIICBA: phosphorylated by HPr
  • MtlA, MtlF: mannitol permease: phosphorylated by HPr
  • GmuA, GmuB, GmuC: galactomannan permease: phosphorylated by HPr
  • TreP: trehalose permease: phosphorylated by HPr
  • MalP: maltose permease: phosphorylated by HPr
  • FruA: fructose permease: phosphorylated by HPr
  • ManP: mannose permease: phosphorylated by HPr
  • LevD, LevE, LevF, LevG: fructose permease: phosphorylated by HPr
  • LicA, LicB, LicC: lichenan permease: phosphorylated by HPr
  • BglP: ß-glucoside permease
  • YpqE: unknown EIIA component: phosphorylated by HPr
  • YyzE: unknown PTS protein

• Non-PTS proteins controlled by PTS-dependent phosphorylation
  • GlpK: phosphorylated by HPr
  • GlcT: phosphorylated by HPr and by PtsG
  • LicT: phosphorylated by HPr and likely by BglP
  • SacT: phosphorylated by HPr and likely by SacP
  • SacY: phosphorylated by HPr and likely by SacY
  • LevR: phosphorylated by HPr and by LevE
  • LicR: phosphorylated by HPr and likely by LicB
  • ManR: phosphorylated by HPr and likely by ManP
  • MtlR: phosphorylated by HPr and likely by MtlA

• Protein kinases of two-component systems

These kinases are dimeric proteins. Phosphorylation occurs from one subunit to the other (not quite an autophosphorylation).

• • ComP
  • DegS
  • DesK
  • LiaS
  • YdfH
  • YfiJ
  • YhcY
  • YvfT
  • YxjM
  • BceS
  • CssS
  • NatK
  • PhoR
  • ResE
  • WalK
  • YbdK
  • YcbM
  • YclK
  • YkoH
  • YrkO
  • YvcQ
  • YvrG
  • YxdK
  • CheA
  • CitS
  • DctS
  • GlnK
  • MalK
  • LytS
  • YesM
  • YwpD
  • KinA
  • KinB
  • KinC
  • KinD
  • KinE
  • Spo0B: part of the phosphorelay, phosphorylated by Spo0F

Proteins closely related to the PTS

• Crh: HPr-like protein with exculsively regulatory functions (His-15 is not conserved
• HprK: HPr-kinase, key factor for carbon catabolite repression

Related Lists

• two-component systems
• PTS
• PRD-containing transcription factors
• phosphorelay
• response regulator aspartate phosphatases