RibA

• Description: GTP cyclohydrolase II/ 3,4-dihydroxy-2-butanone 4-phosphate synthase
  
  

Gene name	ribA
Synonyms
Essential	no
Product	GTP cyclohydrolase II/ 3,4-dihydroxy-2-butanone 4-phosphate synthase
Function	riboflavin biosynthesis
MW, pI	43 kDa, 5.582
Gene length, protein length	1194 bp, 398 aa
Immediate neighbours	ribH, ribE
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag:

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• Swiss prot entry: P17620

• KEGG entry: BSU23260

• E.C. number: 3.5.4.25

Expression and regulation

• Operon: ypuE-ribD-ribE-ribA-ribH-ribT PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

V N Mironov, A S Kraev, M L Chikindas, B K Chernov, A I Stepanov, K G Skryabin
Functional organization of the riboflavin biosynthesis operon from Bacillus subtilis SHgw.
Mol Gen Genet: 1994, 242(2);201-8
[PubMed:8159171] [WorldCat.org] [DOI] (P p)

V Azevedo, A Sorokin, S D Ehrlich, P Serror
The transcriptional organization of the Bacillus subtilis 168 chromosome region between the spoVAF and serA genetic loci.
Mol Microbiol: 1993, 10(2);397-405
[PubMed:7934830] [WorldCat.org] [DOI] (P p)

1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed