ribD

ribD
168

5-amino-6-(5-phosphoribosylamino)uracil reductase

Locus
BSU_23280
Molecular weight
39.15 kDa
Isoelectric point
6.08
Protein length
361 aaSequenceBlast
Gene length
1086 bpSequenceBlast
Function
riboflavin biosynthesis
Product
5-amino-6-(5-phosphoribosylamino)uracil reductase
Essential
no
E.C.
3.5.4.26
Synonyms
ribD, ribG
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1985 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,430,258  2,431,343
The protein
Catalyzed reaction/ biological activity
2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + H+ + H2O --> 5-amino-6-(5-phospho-D-ribosylamino)uracil + NH4+ (according to UniProt)
5-amino-6-(5-phospho-D-ribitylamino)uracil + NADP+ --> 5-amino-6-(5-phospho-D-ribosylamino)uracil + H+ + NADPH (according to UniProt)
Protein family
N-terminal part: Cytidine and deoxycytidylate deaminase family (according to UniProt)
C-terminal part: HTP reductase family (single member, according to UniProt)
Domains
CMP/dCMP-type deaminase domain (aa 1–122) (according to UniProt)
Structure
3EX8 (PDB) PubMed
P17618 (AlphaFold)
Localization
cytoplasm PubMed
Expression and Regulation
Operons
Genes
ribD-ribE-ribA-ribH-ribT
Description
PubMed
Regulation
expressed in the absence of FMN (FMN-box) PubMed
binding of RibR to the FMN-box riboswitch can enforce expression even in the presence of FMN PubMed
the FMN-box RNA is degraded by RNase Y PubMed
Regulatory mechanism
FMN-box: RNA switch, via FMN-box in the presence of FMN or FMNH2, this is counter-acted upon binding of RibR, in FMN-box
RibR: antitermination, PubMed, in ribR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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ribD→ribT

2025-03-26 01:47:48

Jstuelk

122

3D660447137821317811200D29A5FC04A41E938E

05C8A4E892E7DB3D953FE72E8765638644759326

Biological materials
Mutant
BKE23280 (ribD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGTTTCCCTCCCCTCT,  downstream forward: _UP4_CCGACAAAGGAATAGGATGG
BKK23280 (ribD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGTTTCCCTCCCCTCT,  downstream forward: _UP4_CCGACAAAGGAATAGGATGG
References
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3DFCF7C5FE15C9A3E9E7E4B0DA8E926B85DBD180

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Time of last update: 2025-04-02 09:09:47

Author of last update: Jstuelk