ribH

ribH
168

riboflavin synthase (beta subunit)

Locus
BSU_23250
Molecular weight
16.14 kDa
Isoelectric point
5.2
Protein length
154 aaSequenceBlast
Gene length
465 bpSequenceBlast
Function
riboflavin biosynthesis
Product
riboflavin synthase (beta subunit)
Essential
no
E.C.
2.5.1.9
Synonyms
ribH
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0054 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,427,892  2,428,356
The protein
Catalyzed reaction/ biological activity
(2S)-2-hydroxy-3-oxobutyl phosphate + 5-amino-6-(D-ribitylamino)uracil --> 6,7-dimethyl-8-(1-D-ribityl)lumazine + H+ + 2 H2O + phosphate (according to UniProt)
Protein family
DMRL synthase family (single member, according to UniProt)
Structure
1ZIS (PDB), 1RVV (PDB) (the RibE)3-(RibH)60 lumazine synthase/riboflavin synthase complex) PubMed
P11998 (AlphaFold)
Modification
acetylyated on K29 by RibT PubMed
Localization
cytoplasm PubMed
Expression and Regulation
Operons
Genes
ribD-ribE-ribA-ribH-ribT
Description
PubMed
Regulation
expressed in the absence of FMN (FMN-box) PubMed
binding of RibR to the FMN-box riboswitch can enforce expression even in the presence of FMN PubMed
the FMN-box RNA is degraded by RNase Y PubMed
Regulatory mechanism
FMN-box: RNA switch, via FMN-box in the presence of FMN or FMNH2, this is counter-acted upon binding of RibR, in FMN-box
RibR: antitermination, PubMed, in ribR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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ribD→ribT

2025-03-26 01:47:48

Jstuelk

122

3D660447137821317811200D29A5FC04A41E938E

05C8A4E892E7DB3D953FE72E8765638644759326

Biological materials
Mutant
BKE23250 (ribH::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATGATTCCCATCCTTTT,  downstream forward: _UP4_TAATTTGCTGAAAACAGTTT
BKK23250 (ribH::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATGATTCCCATCCTTTT,  downstream forward: _UP4_TAATTTGCTGAAAACAGTTT
References
Reviews
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Original Publications
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2A5DCBFAB51996F0DDEC964D5D178274AF86CE69

Page visits: 3555

Time of last update: 2025-04-01 20:59:01

Author of last update: Jstuelk