FlhG

• Description: GTPase activating protein, activates FlhF, control of flagellar basal body position
  
  

Gene name	flhG
Synonyms	ylxH
Essential	no
Product	GTPase activating protein
Function	activation of FlhF
Gene expression levels in SubtiExpress: flhG
Interactions involving this protein in SubtInteract: FlhG
MW, pI	33 kDa, 9.648
Gene length, protein length	894 bp, 298 aa
Immediate neighbours	flhF, cheB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

motility and chemotaxis, resistance against toxins/ antibiotics

This gene is a member of the following regulons

CodY regulon, DegU regulon, SigD regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU16410

Phenotypes of a mutant

• susceptible to acriflavine and ethidium bromide, and severe growth inhibition as surfactin concentration increased up to 100ug/ml.

Database entries

• BsubCyc: BSU16410

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • binds FlhF and stimulates its GTPase activity PubMed
  • controls the distance between the flagellar bodies PubMed

• Protein family: View classification (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • FlhG-FlhF (FlhG binds the GTP-bound homodimer of FlhF) PubMed

• Localization: cytoplasm (Homogeneous) PubMed

Database entries

• BsubCyc: BSU16410

• Structure:

• UniProt:
  • P40742
  • 3SYN (complex with FlhF) PubMed

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed
  • ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed

• Expression browser: ylxH PubMed

• Sigma factor:
  • flgB: SigA PubMed, SigD PubMed
  • ylxF: SigD PubMed

• Regulation:
  • repressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed
  • CodY: transcription repression PubMed
  • DegU (non-phosphorylated): transcription activation PubMed
  • DegU-P: transcription repression PubMed
  • DegU-P in complex with SwrA: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: