FliF

• Description: flagellar basal-body M-ring protein, membrane anchor of the basal body
  
  

Gene name	fliF
Synonyms
Essential	no
Product	flagellar basal-body M-ring protein
Function	movement and chemotaxis
Gene expression levels in SubtiExpress: fliF
MW, pI	59 kDa, 4.37
Gene length, protein length	1608 bp, 536 aa
Immediate neighbours	fliE, fliG
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

motility and chemotaxis, membrane proteins

This gene is a member of the following regulons

CodY regulon, SigD regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU16210

Phenotypes of a mutant

• mucoid phenotype due to the overproduction of poly-gamma-glutamate PubMed

Database entries

• BsubCyc: BSU16210

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • required for correct localization of FliM PubMed

• Protein family: fliF family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane PubMed

Database entries

• BsubCyc: BSU16210

• Structure:

• UniProt: P23447

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed

• Expression browser: fliF PubMed

• Sigma factor: SigA PubMed, SigD PubMed

• Regulation:
  • repressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed
  • CodY: transcription repression PubMed
  • DegU (non-phosphorylated): transcription activation PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 68 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 595 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: