FliY

• Description: flagellar motor switch protein
  
  

Gene name	fliY
Synonyms	cheD
Essential	no
Product	flagellar motor switch protein
Function	movement and chemotaxis
Gene expression levels in SubtiExpress: fliY
MW, pI	40 kDa, 4.117
Gene length, protein length	1134 bp, 378 aa
Immediate neighbours	fliM, cheY
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

motility and chemotaxis, membrane proteins, phosphoproteins

This gene is a member of the following regulons

CodY regulon, SigD regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU16320

Database entries

• BsubCyc: BSU16320

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: fliN/mopA/spaO family (according to Swiss-Prot) cheC family

• Paralogous protein(s): CheC, FliM

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-251 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• BsubCyc: BSU16320

• Structure:

• UniProt: P24073

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed
  • ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed

• Expression browser: fliY PubMed

• Sigma factor:
  • flgB: SigA PubMed, SigD PubMed
  • ylxF: SigD PubMed

• Regulation:
  • repressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed
  • CodY: transcription repression PubMed
  • DegU (non-phosphorylated): transcription activation PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 1488 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1885 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: