BkdB

• Description: 2-oxoisovalerate dehydrogenase (E2 subunit, lipoamide acyltransferase)
  
  

Gene name	bkdB
Synonyms	bfmBB, bfmB2, bkd
Essential	no
Product	2-oxoisovalerate dehydrogenase (E2 subunit, lipoamide acyltransferase)
Function	utilization of branched-chain keto acids
Gene expression levels in SubtiExpress: bkdB
Metabolic function and regulation of this protein in SubtiPathways: Lipid synthesis, Ile, Leu, Val
MW, pI	45 kDa, 5.301
Gene length, protein length	1272 bp, 424 aa
Immediate neighbours	bmrR, bkdAB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of amino acids

This gene is a member of the following regulons

BkdR regulon, CodY regulon, SigL regulon

The gene

Basic information

• Locus tag: BSU24030

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 2-methylpropanoyl-CoA + enzyme N(6)-(dihydrolipoyl)lysine = CoA + enzyme N(6)-(S-(2-methylpropanoyl)dihydrolipoyl)lysine (according to Swiss-Prot)

• Protein family: 2-oxoacid dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s): AcoC, OdhB, PdhC

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):
  • lipoic acid

• Effectors of protein activity:

• Interactions:

• Localization: Nucleoid (Heterogeneous) PubMed

Database entries

• Structure:

• UniProt: P37942

• KEGG entry: [3]

• E.C. number: 2.3.1.168

Expression and regulation

• Operon: ptb-bcd-buk-lpdV-bkdAA-bkdAB-bkdB PubMed

• Expression browser: bkdB PubMed

• Sigma factor: SigL PubMed

• Regulation:
  • induced in the presence of isoleucine or valine (BkdR) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed
  • BkdR: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: