Ptb

• Description: phosphate butyryltransferase
  
  

Gene name	ptb
Synonyms	yqiS, bkd
Essential	no
Product	phosphate butyryltransferase
Function	utilization of branched-chain keto acids
Gene expression levels in SubtiExpress: ptb
Metabolic function and regulation of this protein in SubtiPathways: Lipid synthesis, Ile, Leu, Val
MW, pI	31 kDa, 7.936
Gene length, protein length	897 bp, 299 aa
Immediate neighbours	bcd, bkdR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of amino acids

This gene is a member of the following regulons

BkdR regulon, CodY regulon, SigL regulon

The gene

Basic information

• Locus tag: BSU24090

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Butanoyl-CoA + phosphate = CoA + butanoylphosphate (according to Swiss-Prot)

• Protein family: phosphate acetyltransferase and butyryltransferase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1YCO (from Enterococcus faecalis v583, 36% identity, 56% similarity)

• UniProt: P54530

• KEGG entry: [3]

• E.C. number: 2.3.1.19

Expression and regulation

• Operon: ptb-bcd-buk-lpdV-bkdAA-bkdAB-bkdB PubMed

• Expression browser: ptb PubMed

• Sigma factor: SigL PubMed

• Regulation:
  • induced in the presence of isoleucine or valine (BkdR) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed
  • BkdR: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: