Difference between revisions of "SigD"

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(Biological materials)
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** 1A716 ( ''sigD''::''cat''), {{PubMed|2832368}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A716&Search=1A716 BGSC]
 
** 1A716 ( ''sigD''::''cat''), {{PubMed|2832368}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A716&Search=1A716 BGSC]
 
** DS6420 (marker-less  in NCIB3610) {{PubMed|22329926}}
 
** DS6420 (marker-less  in NCIB3610) {{PubMed|22329926}}
** TB188 ''amyE''::Phy-''sfgfp'' (marker-less in NCIB3610 with constitutive expressed ''sfgfp'') {{PubMed|26122431}}
 
** TB204 ''amyE''::Phy-''mKATE2'' (marker-less in NCIB3610 with constitutive expressed ''mKATE2'') {{PubMed|26122431}}
 
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 14:47, 3 July 2015

Gene name sigD
Synonyms flaB
Essential no
Product RNA polymerase sigma factor SigD
Function regulation of flagella, motility, chemotaxis and autolysis
Gene expression levels in SubtiExpress: sigD
Interactions involving this protein in SubtInteract: SigD
MW, pI 29 kDa, 5.167
Gene length, protein length 762 bp, 254 aa
Immediate neighbours cheD, swrB
Sequences Protein DNA DNA_with_flanks
Genetic context
SigD context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SigD expression.png















Categories containing this gene/protein

transcription, sigma factors and their control, motility and chemotaxis

This gene is a member of the following regulons

CodY regulon, DegU regulon, SigD regulon, Spo0A regulon

The SigD regulon

The gene

Basic information

  • Locus tag: BSU16470

Phenotypes of a mutant

  • Inactivation of alsR, sigD and sigW increases competitive fitness of Bacillus subtilis under non-sporulating conditions PubMed
  • mucoid phenotype due to the overproduction of poly-gamma-glutamate (because of the loss of motA-motB expression) PubMed
  • not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: sigma-70 factor family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity: interaction with FlgM inhibits SigD activity PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Additional information:

Biological materials

  • Mutant:
    • 1A716 ( sigD::cat), PubMed, available at BGSC
    • DS6420 (marker-less in NCIB3610) PubMed
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • FLAG-tag construct: GP948 (ermC, based on pGP1087), available in the Stülke lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews


The SigD regulon


Original publications