Difference between revisions of "SigD"
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= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
{{SubtiWiki regulon|[[CodY regulon]]}}, | {{SubtiWiki regulon|[[CodY regulon]]}}, | ||
+ | {{SubtiWiki regulon|[[DegU regulon]]}}, | ||
{{SubtiWiki regulon|[[SigD regulon]]}}, | {{SubtiWiki regulon|[[SigD regulon]]}}, | ||
{{SubtiWiki regulon|[[Spo0A regulon]]}} | {{SubtiWiki regulon|[[Spo0A regulon]]}} | ||
Line 129: | Line 130: | ||
** [[CodY]]: transcription repression {{PubMed|12730172}} | ** [[CodY]]: transcription repression {{PubMed|12730172}} | ||
** [[DegU]] (non-phosphorylated): transcription activation {{PubMed|17850253}} | ** [[DegU]] (non-phosphorylated): transcription activation {{PubMed|17850253}} | ||
+ | ** [[DegU]]-P: transcription repression {{PubMed|24386445}} | ||
+ | ** [[DegU]]-P in complex with [[SwrA]]: transcription activation {{PubMed|24386445}} | ||
* '''Additional information:''' | * '''Additional information:''' | ||
Line 161: | Line 164: | ||
==Original publications== | ==Original publications== | ||
− | <pubmed> 22344650 22329926,21097624,22956758 21856853 8955328,7708689,9648743, 8169223,9096206,15175317, 2498284,16357223, 3127378, 9144176,18820022,7602586,2832368,8195064,11987133,,14651647, 9335309 9657996,8157612,15175317 10809682 8932324 20233303 17850253 10207036 16428420 24296669 25099370 </pubmed> | + | <pubmed> 22344650 22329926,21097624,22956758 21856853 8955328,7708689,9648743, 8169223,9096206,15175317, 2498284,16357223, 3127378, 9144176,18820022,7602586,2832368,8195064,11987133,,14651647, 9335309 9657996,8157612,15175317 10809682 8932324 20233303 17850253 10207036 16428420 24296669 25099370 24386445</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] |
Revision as of 15:52, 29 December 2014
- Description: RNA polymerase sigma factor SigD
Gene name | sigD |
Synonyms | flaB |
Essential | no |
Product | RNA polymerase sigma factor SigD |
Function | regulation of flagella, motility, chemotaxis and autolysis |
Gene expression levels in SubtiExpress: sigD | |
Interactions involving this protein in SubtInteract: SigD | |
MW, pI | 29 kDa, 5.167 |
Gene length, protein length | 762 bp, 254 aa |
Immediate neighbours | cheD, swrB |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
transcription, sigma factors and their control, motility and chemotaxis
This gene is a member of the following regulons
CodY regulon, DegU regulon, SigD regulon, Spo0A regulon
The SigD regulon
The gene
Basic information
- Locus tag: BSU16470
Phenotypes of a mutant
- Inactivation of alsR, sigD and sigW increases competitive fitness of Bacillus subtilis under non-sporulating conditions PubMed
- mucoid phenotype due to the overproduction of poly-gamma-glutamate (because of the loss of motA-motB expression) PubMed
Database entries
- BsubCyc: BSU16470
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: sigma-70 factor family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
Database entries
- BsubCyc: BSU16470
- Structure:
- UniProt: P10726
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed
- ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed
- sigD-swrB PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Sampriti Mukherjee, Daniel B Kearns
The structure and regulation of flagella in Bacillus subtilis.
Annu Rev Genet: 2014, 48;319-40
[PubMed:25251856]
[WorldCat.org]
[DOI]
(I p)
Jan-Willem Veening, Oscar P Kuipers
Gene position within a long transcript as a determinant for stochastic switching in bacteria.
Mol Microbiol: 2010, 76(2);269-72
[PubMed:20233302]
[WorldCat.org]
[DOI]
(I p)
The SigD regulon
Masakuni Serizawa, Hiroki Yamamoto, Hirotake Yamaguchi, Yasutaro Fujita, Kazuo Kobayashi, Naotake Ogasawara, Junichi Sekiguchi
Systematic analysis of SigD-regulated genes in Bacillus subtilis by DNA microarray and Northern blotting analyses.
Gene: 2004, 329;125-36
[PubMed:15033535]
[WorldCat.org]
[DOI]
(P p)
L M Márquez, J D Helmann, E Ferrari, H M Parker, G W Ordal, M J Chamberlin
Studies of sigma D-dependent functions in Bacillus subtilis.
J Bacteriol: 1990, 172(6);3435-43
[PubMed:2111808]
[WorldCat.org]
[DOI]
(P p)
Original publications