Difference between revisions of "SrfAD"

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|style="background:#ABCDEF;" align="center"|'''Function''' || antibiotic synthesis
 
|style="background:#ABCDEF;" align="center"|'''Function''' || antibiotic synthesis
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU03520 srfAD]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU03520 srfAD]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 27 kDa, 5.22   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 27 kDa, 5.22   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[srfAC]]'', ''[[ycxA]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[srfAC]]'', ''[[ycxA]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12146&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU03520 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU03520 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU03520 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:srfAD_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:srfAD_context.gif]]

Revision as of 12:07, 13 May 2013

  • Description: surfactin synthetase / competence

Gene name srfAD
Synonyms comL
Essential no
Product surfactin synthetase / competence
Function antibiotic synthesis
Gene expression levels in SubtiExpress: srfAD
MW, pI 27 kDa, 5.22
Gene length, protein length 726 bp, 242 aa
Immediate neighbours srfAC, ycxA
Sequences Protein DNA Advanced_DNA
Genetic context
SrfAD context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SrfAD expression.png
























Categories containing this gene/protein

miscellaneous metabolic pathways, biosynthesis of antibacterial compounds

This gene is a member of the following regulons

Abh regulon, CodY regulon, ComA regulon, PerR regulon, Spx regulon

The gene

Basic information

  • Locus tag: BSU03520

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: thioesterase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
    • repressed during hydrogen peroxide stress (PerR) PubMed
    • expressed at high cell density (ComA) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Massimiliano Marvasi, Pieter T Visscher, Lilliam Casillas Martinez
Exopolymeric substances (EPS) from Bacillus subtilis: polymers and genes encoding their synthesis.
FEMS Microbiol Lett: 2010, 313(1);1-9
[PubMed:20735481] [WorldCat.org] [DOI] (I p)

Original publications

Additional publications: PubMed