FliF

• Description: flagellar basal-body M-ring protein, membrane anchor of the basal body
  
  

Gene name	fliF
Synonyms
Essential	no
Product	flagellar basal-body M-ring protein
Function	movement and chemotaxis
Gene expression levels in SubtiExpress: fliF
Interactions involving this protein in SubtInteract: FliF
MW, pI	59 kDa, 4.37
Gene length, protein length	1608 bp, 536 aa
Immediate neighbours	fliE, fliG
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

motility and chemotaxis, membrane proteins

This gene is a member of the following regulons

CodY regulon, DegU regulon, SigD regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU16210

Phenotypes of a mutant

• mucoid phenotype due to the overproduction of poly-gamma-glutamate PubMed
• no secretion of FlgM, permanent inhibition of SigD PubMed
• not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation PubMed

Database entries

• BsubCyc: BSU16210

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • required for correct localization of FliM PubMed

• Protein family: fliF family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • FliF-FliG PubMed

• Localization:
  • cell membrane PubMed

Database entries

• BsubCyc: BSU16210

• Structure:

• UniProt: P23447

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: part of the fla-che operon (see there for regulation)
  • flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed

• Expression browser: fliF PubMed

• Sigma factor: SigA PubMed, SigD PubMed

• Regulation: see fla-che operon

• Regulatory mechanism: see fla-che operon

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 68 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 595 PubMed

Biological materials

• Mutant:
  • DS7080 (marker-less in NCIB3610) PubMed
  • TB192 amyE::Phy-sfgfp (marker-less in NCIB3610 with constitutive expressed sfgfp) PubMed
  • TB208 amyE::Phy-mKATE2 (marker-less in NCIB3610 with constitutive expressed mKATE2) PubMed

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Theresa Hölscher, Benjamin Bartels, Yu-Cheng Lin, Ramses Gallegos-Monterrosa, Alexa Price-Whelan, Roberto Kolter, Lars E P Dietrich, Ákos T Kovács
Motility, Chemotaxis and Aerotaxis Contribute to Competitiveness during Bacterial Pellicle Biofilm Development.
J Mol Biol: 2015, 427(23);3695-3708
[PubMed:26122431] [WorldCat.org] [DOI] (I p)

Yuichi Hakumai, Kouko Shimomoto, Makoto Ashiuchi
Extra-chromosomal DNA maintenance in Bacillus subtilis, dependence on flagellation factor FliF and moonlighting mediator EdmS.
Biochem Biophys Res Commun: 2015, 460(4);1059-62
[PubMed:25843804] [WorldCat.org] [DOI] (I p)

Rebecca A Calvo, Daniel B Kearns
FlgM is secreted by the flagellar export apparatus in Bacillus subtilis.
J Bacteriol: 2015, 197(1);81-91
[PubMed:25313396] [WorldCat.org] [DOI] (I p)

Serena Mordini, Cecilia Osera, Simone Marini, Francesco Scavone, Riccardo Bellazzi, Alessandro Galizzi, Cinzia Calvio
The role of SwrA, DegU and P(D3) in fla/che expression in B. subtilis.
PLoS One: 2013, 8(12);e85065
[PubMed:24386445] [WorldCat.org] [DOI] (I e)

Jia Mun Chan, Sarah B Guttenplan, Daniel B Kearns
Defects in the flagellar motor increase synthesis of poly-γ-glutamate in Bacillus subtilis.
J Bacteriol: 2014, 196(4);740-53
[PubMed:24296669] [WorldCat.org] [DOI] (I p)

Sarah B Guttenplan, Sidney Shaw, Daniel B Kearns
The cell biology of peritrichous flagella in Bacillus subtilis.
Mol Microbiol: 2013, 87(1);211-29
[PubMed:23190039] [WorldCat.org] [DOI] (I p)

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Kazuo Kobayashi
Gradual activation of the response regulator DegU controls serial expression of genes for flagellum formation and biofilm formation in Bacillus subtilis.
Mol Microbiol: 2007, 66(2);395-409
[PubMed:17850253] [WorldCat.org] [DOI] (P p)

H Werhane, P Lopez, M Mendel, M Zimmer, G W Ordal, L M Márquez-Magaña
The last gene of the fla/che operon in Bacillus subtilis, ylxL, is required for maximal sigmaD function.
J Bacteriol: 2004, 186(12);4025-9
[PubMed:15175317] [WorldCat.org] [DOI] (P p)

Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647] [WorldCat.org] [DOI] (P p)

W Estacio, S S Anna-Arriola, M Adedipe, L M Márquez-Magaña
Dual promoters are responsible for transcription initiation of the fla/che operon in Bacillus subtilis.
J Bacteriol: 1998, 180(14);3548-55
[PubMed:9657996] [WorldCat.org] [DOI] (P p)

L M Márquez-Magaña, M J Chamberlin
Characterization of the sigD transcription unit of Bacillus subtilis.
J Bacteriol: 1994, 176(8);2427-34
[PubMed:8157612] [WorldCat.org] [DOI] (P p)

A R Zuberi, C Ying, D S Bischoff, G W Ordal
Gene-protein relationships in the flagellar hook-basal body complex of Bacillus subtilis: sequences of the flgB, flgC, flgG, fliE and fliF genes.
Gene: 1991, 101(1);23-31
[PubMed:1905667] [WorldCat.org] [DOI] (P p)