CheB

• Description: MCP-glutamate methylesterase / two-component response regulator-like
  
  

Gene name	cheB
Synonyms	cheL
Essential	no
Product	MCP-glutamate methylesterase / two-component response regulator-like
Function	motility and chemotaxis
Gene expression levels in SubtiExpress: cheB
Interactions involving this protein in SubtInteract: CheB
MW, pI	38 kDa, 8.119
Gene length, protein length	1071 bp, 357 aa
Immediate neighbours	flhG, cheA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

motility and chemotaxis, phosphoproteins

This gene is a member of the following regulons

CodY regulon, DegU regulon, SigD regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU16420

Phenotypes of a mutant

• not essential for pellicle biofilm formation and mutant has similar fitness to the wild-type strain when competed during pellicle formation PubMed

Database entries

• BsubCyc: BSU16420

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Protein L-glutamate O(5)-methyl ester + H₂O = protein L-glutamate + methanol (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated on a Asp residue by CheA PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU16420

• Structure:

• UniProt: Q05522

• KEGG entry: [3]

• E.C. number: 3.1.1.61

Expression and regulation

• Operon: part of the fla-che operon
  • flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed
  • ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed

• Expression browser: cheB PubMed

• Sigma factor:
  • flgB: SigA PubMed, SigD PubMed
  • ylxF: SigD PubMed

• Regulation: see fla-che operon

• Regulatory mechanism: see fla-che operon

• Additional information:
  • in minimal medium, CheB is present with 2,100 +/- 400 molecules per cell PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 92 PubMed

Biological materials

• Mutant:
  • DS7306 (marker-less in NCIB3610) PubMed
  • TB194 amyE::Phy-sfgfp (marker-less in NCIB3610 with constitutive expressed sfgfp) PubMed
  • TB209 amyE::Phy-mKATE2 (marker-less in NCIB3610 with constitutive expressed mKATE2) PubMed

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Daniel Kearns