Difference between revisions of "FliF"

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(Expression and regulation)
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=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' ''[[flgB]]-[[flgC]]-[[fliE]]-[[fliF]]-[[fliG]]-[[fliH]]-[[fliI]]-[[fliJ]]-[[ylxF]]-[[fliK]]-[[flgD]]-[[flgE]]-[[fliL]]-[[fliM]]-[[fliY]]-[[cheY]]-[[fliZ]]-[[fliP]]-[[fliQ]]-[[fliR]]-[[flhB]]-[[flhA]]-[[flhF]]-[[flhG]]-[[cheB]]-[[cheA]]-[[cheW]]-[[cheC]]-[[cheD]]-[[sigD]]-[[swrB]]'' {{PubMed|9657996,8157612,15175317}}
+
* '''Operon:''' part of the [[fla-che operon]] (see there for regulation)
 +
** ''[[flgB]]-[[flgC]]-[[fliE]]-[[fliF]]-[[fliG]]-[[fliH]]-[[fliI]]-[[fliJ]]-[[ylxF]]-[[fliK]]-[[flgD]]-[[flgE]]-[[fliL]]-[[fliM]]-[[fliY]]-[[cheY]]-[[fliZ]]-[[fliP]]-[[fliQ]]-[[fliR]]-[[flhB]]-[[flhA]]-[[flhF]]-[[flhG]]-[[cheB]]-[[cheA]]-[[cheW]]-[[cheC]]-[[cheD]]-[[sigD]]-[[swrB]]'' {{PubMed|9657996,8157612,15175317}}
  
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=fliF_1692496_1694106_1 fliF] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=fliF_1692496_1694106_1 fliF] {{PubMed|22383849}}
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* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|9657996}}, [[SigD]] {{PubMed|9657996}}
 
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|9657996}}, [[SigD]] {{PubMed|9657996}}
  
* '''Regulation:'''  
+
* '''Regulation:''' see [[fla-che operon]]
** repressed under conditions that trigger sporulation ([[Spo0A]]) {{PubMed|14651647}}
 
  
* '''Regulatory mechanism:'''  
+
* '''Regulatory mechanism:''' see [[fla-che operon]]
** [[Spo0A]]: transcription repression {{PubMed|14651647}}
 
** [[CodY]]: transcription repression {{PubMed|12730172}}
 
** [[DegU]] (non-phosphorylated): transcription activation {{PubMed|17850253}}
 
** [[DegU]]-P: transcription repression {{PubMed|24386445}}
 
** [[DegU]]-P in complex with [[SwrA]]: transcription activation {{PubMed|24386445}}
 
  
 
* '''Additional information:'''
 
* '''Additional information:'''

Revision as of 14:09, 12 February 2015

  • Description: flagellar basal-body M-ring protein, membrane anchor of the basal body

Gene name fliF
Synonyms
Essential no
Product flagellar basal-body M-ring protein
Function movement and chemotaxis
Gene expression levels in SubtiExpress: fliF
Interactions involving this protein in SubtInteract: FliF
MW, pI 59 kDa, 4.37
Gene length, protein length 1608 bp, 536 aa
Immediate neighbours fliE, fliG
Sequences Protein DNA DNA_with_flanks
Genetic context
FliF context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
FliF expression.png















Categories containing this gene/protein

motility and chemotaxis, membrane proteins

This gene is a member of the following regulons

CodY regulon, DegU regulon, SigD regulon, Spo0A regulon

The gene

Basic information

  • Locus tag: BSU16210

Phenotypes of a mutant

  • mucoid phenotype due to the overproduction of poly-gamma-glutamate PubMed
  • no secretion of FlgM, permanent inhibition of SigD PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: fliF family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 68 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 595 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References