Difference between revisions of "CheW"
| Line 128: | Line 128: | ||
** number of protein molecules per cell (minimal medium with glucose and ammonium): 840 {{PubMed|24696501}} | ** number of protein molecules per cell (minimal medium with glucose and ammonium): 840 {{PubMed|24696501}} | ||
** number of protein molecules per cell (complex medium with amino acids, without glucose): 1423 {{PubMed|24696501}} | ** number of protein molecules per cell (complex medium with amino acids, without glucose): 1423 {{PubMed|24696501}} | ||
| + | ** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1996 {{PubMed|21395229}} | ||
| + | ** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 1296 {{PubMed|21395229}} | ||
| + | ** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 699 {{PubMed|21395229}} | ||
=Biological materials = | =Biological materials = | ||
| − | |||
* '''Mutant:''' | * '''Mutant:''' | ||
Revision as of 14:04, 17 April 2014
- Description: modulation of CheA activity in response to attractants, scaffold protein: facilitates coupling between CheA and receptors
| Gene name | cheW |
| Synonyms | |
| Essential | no |
| Product | CheA modulator |
| Function | control of CheA activity |
| Gene expression levels in SubtiExpress: cheW | |
| MW, pI | 17 kDa, 4.422 |
| Gene length, protein length | 468 bp, 156 aa |
| Immediate neighbours | cheA, cheC |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
CodY regulon, SigD regulon, Spo0A regulon
The gene
Basic information
- Locus tag: BSU16440
Phenotypes of a mutant
Database entries
- BsubCyc: BSU16440
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s): CheV (N-terminal domain)
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- cytoplasm (according to Swiss-Prot)
- predominantly present at the cell poles PubMed
Database entries
- BsubCyc: BSU16440
- Structure:
- UniProt: P39802
- KEGG entry: [3]
- E.C. number:
Additional information
- subject to Clp-dependent proteolysis upon glucose starvation PubMed
Expression and regulation
- Operon:
- Regulatory mechanism:
- Additional information:
- subject to Clp-dependent proteolysis upon glucose starvation PubMed
- in minimal medium, CheW is present with 2,100 +/- 430 molecules per cell PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium): 840 PubMed
- number of protein molecules per cell (complex medium with amino acids, without glucose): 1423 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1996 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 1296 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 699 PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Original publications
