Difference between revisions of "CodY"

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* '''Additional information:'''  
 
* '''Additional information:'''  
 
** the intracellular concentration of CodY is about 2.5 myM  (according to {{PubMed|20408793}})
 
** the intracellular concentration of CodY is about 2.5 myM  (according to {{PubMed|20408793}})
 +
** number of protein molecules per cell (minimal medium with glucose and ammonium): 955 {{PubMed|24696501}}
 +
** number of protein molecules per cell (complex medium with amino acids, without glucose): 3409 {{PubMed|24696501}}
  
 
=Biological materials =
 
=Biological materials =

Revision as of 09:46, 17 April 2014

  • Description: regulation of a large regulon (more than 100 genes and operons) in response to branched-chain amino acid limitation

Gene name codY
Synonyms
Essential no
Product transcriptional pleiotropic repressor
Function regulation of a large regulon in response to

branched-chain amino acid limitation

to the presence of branched-chain amino acids

Gene expression levels in SubtiExpress: codY
Interactions involving this protein in SubtInteract: CodY
Metabolic function and regulation of this protein in SubtiPathways:
codY
MW, pI 28 kDa, 4.75
Gene length, protein length 777 bp, 259 aa
Immediate neighbours clpY, flgB
Sequences Protein DNA DNA_with_flanks
Genetic context
CodY context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CodY expression.png















Categories containing this gene/protein

transcription factors and their control, regulators of core metabolism, phosphoproteins

This gene is a member of the following regulons

CodY regulon

The CodY regulon

The gene

Basic information

  • Locus tag: BSU16170

Phenotypes of a mutant

  • no swarming motility on B medium. PubMed
  • the mutation suppresses the mucoid phenotype of motA or motB mutants due to loss of DegU phosphorylation and concomitant reduced expression of the capB-capC-capA-capE operon PubMed
  • inactivation of codY suppresses the requirement of a relA sasA sasB triple mutant for branched chain amino acids, methionine and threonine PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: codY family (according to Swiss-Prot)
  • Paralogous protein(s):

Genes/ operons controlled by CodY

Extended information on the protein

  • Kinetic information:
  • Domains: contains a GAF domain (ligand binding domain)
  • Modification: phosphorylation on Ser-215 PubMed
  • Effectors of protein activity: GTP and branched chained amino acids (BCAA) increase the affinity of CodY for its DNA target sequences PubMed

Database entries

  • Structure: 2B0L (C-terminal DNA-binding domain), 2GX5 (N-terminal Gaf domain)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • Regulatory mechanism:
  • Additional information:
    • the intracellular concentration of CodY is about 2.5 myM (according to PubMed)
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 955 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 3409 PubMed

Biological materials

  • Expression vector:
    • for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP245, available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Linc Sonenshein, Tufts University, Boston, MA, USA Homepage

Tony Wilkinson, York University, U.K. homepage

Oscar Kuipers, University of Groningen, The Netherlands Homepage

Your additional remarks

References

Reviews


The CodY regulon

Original Publications