Difference between revisions of "SsbA"
| Line 84: | Line 84: | ||
* '''Kinetic information:''' | * '''Kinetic information:''' | ||
| − | * '''Domains:''' | + | * '''[[Domains]]:''' |
* '''Modification:''' | * '''Modification:''' | ||
| Line 90: | Line 90: | ||
** phosphorylated on Tyr-82 by [[PtkA]], dephosphorylated by [[PtpZ]] [http://www.ncbi.nlm.nih.gov/sites/entrez/16549871 PubMed] | ** phosphorylated on Tyr-82 by [[PtkA]], dephosphorylated by [[PtpZ]] [http://www.ncbi.nlm.nih.gov/sites/entrez/16549871 PubMed] | ||
| − | * ''' | + | * '''[[Cofactors]]:''' |
* '''Effectors of protein activity:''' Phosphorylation of Ssb increases binding to single-stranded DNA in vitro almost 200-fold [http://www.ncbi.nlm.nih.gov/sites/entrez/16549871 PubMed] | * '''Effectors of protein activity:''' Phosphorylation of Ssb increases binding to single-stranded DNA in vitro almost 200-fold [http://www.ncbi.nlm.nih.gov/sites/entrez/16549871 PubMed] | ||
| Line 128: | Line 128: | ||
=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' ''[[yyaF]]-[[rpsF]]-[[ssbA]]-[[rpsR]]'' {{PubMed|14762004}} | + | * '''Operon:''' |
| + | ** ''[[yyaF]]-[[rpsF]]-[[ssbA]]-[[rpsR]]'' {{PubMed|14762004}} | ||
| + | ** ''[[rpsF]]-[[ssbA]]-[[rpsR]]'' {{PubMed|24310371,22383849}} | ||
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ssbA_4198886_4199404_-1 ssbA] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ssbA_4198886_4199404_-1 ssbA] {{PubMed|22383849}} | ||
* '''[[Sigma factor]]:''' | * '''[[Sigma factor]]:''' | ||
| + | ** ''[[rpsF]]'': [[SigA]] {{PubMed|24310371}} | ||
* '''Regulation:''' | * '''Regulation:''' | ||
| Line 139: | Line 142: | ||
* '''Additional information:''' | * '''Additional information:''' | ||
| + | ** the mRNA contains a structured motif in the 5' UTR upstream of the ''[[rpsF]]'' ORF that is bound by [[RpsF]]-[[RpsR]] heterodimers and may be implicated in autogenous regulation {{PubMed|24310371}} | ||
=Biological materials = | =Biological materials = | ||
| Line 162: | Line 166: | ||
<pubmed> 22976189 22933559 </pubmed> | <pubmed> 22976189 22933559 </pubmed> | ||
== Original publications == | == Original publications == | ||
| − | <pubmed>17853894,19730681 11948146,16479537,11948165 16549871 20122408 14762004 20581116 22054219 22373918 22517742 21859751, 21170359, 21958350,23268446 23536821 23779106</pubmed> | + | <pubmed>17853894,19730681 11948146,16479537,11948165 16549871 20122408 14762004 20581116 22054219 22373918 22517742 21859751, 21170359, 21958350,23268446 23536821 23779106 22383849 24310371 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 18:30, 13 December 2013
- Description: single-strand DNA-binding protein, part of the replisome
| Gene name | ssbA |
| Synonyms | ssb |
| Essential | yes PubMed |
| Product | single-strand DNA-binding protein |
| Function | DNA replication, DNA repair/ recombination |
| Gene expression levels in SubtiExpress: ssbA | |
| Interactions involving this protein in SubtInteract: SsbA | |
| MW, pI | 18 kDa, 4.822 |
| Gene length, protein length | 516 bp, 172 aa |
| Immediate neighbours | rpsR, rpsF |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
DNA replication, DNA repair/ recombination, essential genes, phosphoproteins
This gene is a member of the following regulons
ComK regulon, stringent response
The gene
Basic information
- Locus tag: BSU40900
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s): SsbB
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity: Phosphorylation of Ssb increases binding to single-stranded DNA in vitro almost 200-fold PubMed
- Interactions:
- forms tetramers PubMed
- part of the replisome: PolC-HolA-HolB-DnaX-DnaN-DnaG-DnaC-DnaI-DnaD-SsbA-DnaE-PriA-DnaB PubMed
- SsbA-SbcC PubMed
- SsbA-RecG PubMed
- SsbA-YrvE PubMed
- SsbA-YrvN PubMed
- SsbA (bound to ssDNA) -RecO, this helps to dislodge SsbA and SsbB from ssDNA PubMed
- SsbA-YqiB PubMed
- SsbA-Ung PubMed
- SsbA-YpbB PubMed
- SsbA-YrrC PubMed
- SsbA-DnaN PubMed
- SsbA (bound to ssDNA) -RecO, this helps to dislodge SsbA and SsbB from ssDNA PubMed
- SsbA-YocI according to PubMed
- SsbA-RecQ according to PubMed
Database entries
- UniProt: P37455
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Original publications
