CheY

• Description: two-component response regulator, modulation of flagellar switch bias
  
  

Gene name	cheY
Synonyms	cheB
Essential	no
Product	two-component response regulator
Function	modulation of flagellar switch bias
Gene expression levels in SubtiExpress: cheY
Interactions involving this protein in SubtInteract: CheY
MW, pI	13 kDa, 4.746
Gene length, protein length	360 bp, 120 aa
Immediate neighbours	fliY, fliZ
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transcription factors and their control, motility and chemotaxis, phosphoproteins

This gene is a member of the following regulons

CodY regulon, SigD regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU16330

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated by CheA on an Asp residue
  • dephosphorylated by CheC PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • CheY-P-CheC PubMed
  • CheA-CheY

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P24072

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed
  • ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed

• Expression browser: cheY PubMed

• Sigma factor:
  • flgB: SigA PubMed, SigD PubMed
  • ylxF: SigD PubMed

• Regulation:
  • repressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed
  • CodY: transcription repression PubMed
  • DegU (non-phosphorylated): transcription activation PubMed

• Additional information:
  • in minimal medium, CheY is present with 7,100 +/- 1,000 molecules per cell PubMed

Biological materials

• Mutant:
  • 1A798 ( cheY::cat), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Gerald L Hazelbauer, Wing-Cheung Lai
Bacterial chemoreceptors: providing enhanced features to two-component signaling.
Curr Opin Microbiol: 2010, 13(2);124-32
[PubMed:20122866] [WorldCat.org] [DOI] (I p)

Christopher V Rao, George W Ordal
The molecular basis of excitation and adaptation during chemotactic sensory transduction in bacteria.
Contrib Microbiol: 2009, 16;33-64
[PubMed:19494578] [WorldCat.org] [DOI] (P p)

Christopher V Rao, John R Kirby, Adam P Arkin
Phosphatase localization in bacterial chemotaxis: divergent mechanisms, convergent principles.
Phys Biol: 2005, 2(3);148-58
[PubMed:16224120] [WorldCat.org] [DOI] (I e)

C Fabret, V A Feher, J A Hoch
Two-component signal transduction in Bacillus subtilis: how one organism sees its world.
J Bacteriol: 1999, 181(7);1975-83
[PubMed:10094672] [WorldCat.org] [DOI] (P p)