BkdB

• Description: 2-oxoisovalerate dehydrogenase (E2 subunit, lipoamide acyltransferase)
  
  

Gene name	bkdB
Synonyms	bfmBB, bfmB2, bkd
Essential	no
Product	2-oxoisovalerate dehydrogenase (E2 subunit, lipoamide acyltransferase)
Function	utilization of branched-chain keto acids
MW, pI	45 kDa, 5.301
Gene length, protein length	1272 bp, 424 aa
Immediate neighbours	bmrR, bkdAB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag:

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 2-methylpropanoyl-CoA + enzyme N(6)-(dihydrolipoyl)lysine = CoA + enzyme N(6)-(S-(2-methylpropanoyl)dihydrolipoyl)lysine (according to Swiss-Prot)

• Protein family: 2-oxoacid dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: Nucleoid (Heterogeneous) PubMed

Database entries

• Structure:

• Swiss prot entry:

• KEGG entry: BSU24030

• E.C. number: 2.3.1.168

Expression and regulation

• Operon: ptb-bcd-buk-lpdV-bkdAA-bkdAB-bkdB

• Sigma factor: SigL PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Jean-Christophe Meile, Ling Juan Wu, S Dusko Ehrlich, Jeff Errington, Philippe Noirot
Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory.
Proteomics: 2006, 6(7);2135-46
[PubMed:16479537] [WorldCat.org] [DOI] (P p)

1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed