GyrB

• Description: DNA gyrase (subunit B)
  
  

Gene name	gyrB
Synonyms	novA
Essential	yes PubMed
Product	DNA gyrase (subunit B)
Function	DNA supercoiling, initation of replication cycle and DNA elongation
Gene expression levels in SubtiExpress: GyrB
Interactions involving this protein in SubtInteract: GyrB
MW, pI	71 kDa, 5.378
Gene length, protein length	1914 bp, 638 aa
Immediate neighbours	yaaB, gyrA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA condensation/ segregation, essential genes

The gene

Basic information

• Locus tag: BSU00060

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU00060

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP-dependent breakage, passage and rejoining of double-stranded DNA (according to Swiss-Prot)

• Protein family: type II topoisomerase family (according to Swiss-Prot)

• Paralogous protein(s): ParE

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated on Ser-400 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • GyrA-GyrB

• Localization:
  • nucleoid-associated in actively growing cells PubMed

Database entries

• BsubCyc: BSU00060

• Structure:

• UniProt: P05652

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: yaaA-recF-yaaB-gyrB PubMed

• Expression browser: gyrB PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information: GyrB is subject to Clp-dependent proteolysis upon glucose starvation PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 146 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 416 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Dagmar Klostermeier, Biozentrum Basel, Switzerland homepage

References

Additional references: PubMed