SigA

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Gene name sigA
Synonyms rpoD, crsA
Essential yes PubMed
Product RNA polymerase major sigma factor SigA
Function transcription
Gene expression levels in SubtiExpress: sigA
Interactions involving this protein in SubtInteract: SigA
MW, pI 42 kDa, 4.634
Gene length, protein length 1113 bp, 371 aa
Immediate neighbours cccA, dnaG
Sequences Protein DNA DNA_with_flanks
Genetic context
SigA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SigA expression.png















Categories containing this gene/protein

transcription, sigma factors and their control, essential genes, membrane proteins

This gene is a member of the following regulons

SigD regulon, SigH regulon

The gene

Basic information

  • Locus tag: BSU25200

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: sigma-70 factor family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • An antisense RNA is predicted for sigA PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 802 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 1811 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Benedikt Steuten, Sabine Schneider, Rolf Wagner
6S RNA: recent answers--future questions.
Mol Microbiol: 2014, 91(4);641-8
[PubMed:24308327] [WorldCat.org] [DOI] (I p)

Lakshminarayan M Iyer, L Aravind
Insights from the architecture of the bacterial transcription apparatus.
J Struct Biol: 2012, 179(3);299-319
[PubMed:22210308] [WorldCat.org] [DOI] (I p)


Original publications