PucG

• Description: (S)-ureidoglycine-glyoxylate aminotransferase
  
  

Gene name	pucG
Synonyms	yurG
Essential	no
Product	(S)-ureidoglycine-glyoxylate aminotransferase
Function	purine utilization
Gene expression levels in SubtiExpress: pucG
Metabolic function and regulation of this protein in SubtiPathways: pucG
MW, pI	45 kDa, 5.42
Gene length, protein length	1248 bp, 416 aa
Immediate neighbours	pucA, pucF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of nucleotides

This gene is a member of the following regulons

PucR regulon

The gene

Basic information

• Locus tag: BSU32520

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: transamination between an unstable intermediate ((S)-ureidoglycine) and the end product of purine catabolism (glyoxylate) to yield oxalurate and glycine PubMed

• Protein family: class-V pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3ISL PubMed

• UniProt: O32148

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: pucF-pucG PubMed

• Expression browser: pucG PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced in the presence of purine nucleotides (inducer: allantoin) (PucR) PubMed

• Regulatory mechanism:
  • PucR: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ileana Ramazzina, Roberto Costa, Laura Cendron, Rodolfo Berni, Alessio Peracchi, Giuseppe Zanotti, Riccardo Percudani
An aminotransferase branch point connects purine catabolism to amino acid recycling.
Nat Chem Biol: 2010, 6(11);801-6
[PubMed:20852637] [WorldCat.org] [DOI] (I p)

Lars Beier, Per Nygaard, Hanne Jarmer, Hans H Saxild
Transcription analysis of the Bacillus subtilis PucR regulon and identification of a cis-acting sequence required for PucR-regulated expression of genes involved in purine catabolism.
J Bacteriol: 2002, 184(12);3232-41
[PubMed:12029039] [WorldCat.org] [DOI] (P p)

A C Schultz, P Nygaard, H H Saxild
Functional analysis of 14 genes that constitute the purine catabolic pathway in Bacillus subtilis and evidence for a novel regulon controlled by the PucR transcription activator.
J Bacteriol: 2001, 183(11);3293-302
[PubMed:11344136] [WorldCat.org] [DOI] (P p)