pucF

pucF
168

allantoate amidohydrolase

Locus
BSU_32530
Molecular weight
45.36 kDa
Isoelectric point
5.36
Protein length
412 aaSequenceBlast
Gene length
1239 bpSequenceBlast
Function
purine utilization
Product
allantoate amidohydrolase
Essential
no
Synonyms
pucF, yurH
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0624 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,342,433 → 3,343,671
The protein
Catalyzed reaction/ biological activity
allantoate + 2 H+ + H2O --> (S)-2-ureidoglycine + CO2 + NH4+ (according to UniProt)
Protein family
peptidase M20 family (according to UniProt)
Structure
2IMO (PDB) (from Escherichia coli k12, 47% identity, 63% similarity) PubMed
O32149 (AlphaFold)
Expression and Regulation
Operons
Genes
pucF-pucG
Description
PubMed
Regulation
induced in the presence of purine nucleotides (inducer: allantoin) (PucR) PubMed
Expression
expression is heterogeneous PubMed
Regulatory mechanism
PucR: activation, PubMed, in pucR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

pucF→pucG

2025-03-09 18:05:27

Jstuelk

100

8950B371B49B2D867C39C0645E288618F9712782

C7BE4A3C8762E1DBFB97C41D65DC9339DE1A1137

Biological materials
Mutant
MGNA-A943 (yurH::erm), available at the NBRP B. subtilis, Japan
BKE32530 (ΔpucF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTACTCCCCCCTCTGGC,  downstream forward: _UP4_GCTTACTGATAAAGGAGGAA
BKK32530 (ΔpucF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTACTCCCCCCTCTGGC,  downstream forward: _UP4_GCTTACTGATAAAGGAGGAA
References
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C314CE9D194E8894430368C5F69C0778D45A651C

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Time of last update: 2025-03-12 15:05:49

Author of last update: Jstuelk