Spo0A
Revision as of 08:48, 12 November 2013 by 134.76.70.252 (talk)
- Description: phosphorelay regulator, initiation of sporulation, coordinates DNA replication and initiation of sporulation by binding to sites close to the oriC
Gene name | spo0A |
Synonyms | spo0C, spo0G, spoIIL, sof-1 |
Essential | no |
Product | phosphorelay response regulator |
Function | initiation of sporulation |
Gene expression levels in SubtiExpress: spo0A | |
Interactions involving this protein in SubtInteract: Spo0A | |
Metabolic function and regulation of this protein in SubtiPathways: Biofilm, Nucleotides (regulation), Ammonium/ glutamate, Central C-metabolism,Sugar catabolism, Phosphorelay, Stress, tRNA charging,Lipid synthesis, Protein secretion | |
MW, pI | 29 kDa, 5.989 |
Gene length, protein length | 801 bp, 267 aa |
Immediate neighbours | yqiG, spoIVB |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
transcription factors and their control, phosphorelay, biofilm formation, phosphoproteins
This gene is a member of the following regulons
The Spo0A regulon
The gene
Basic information
- Locus tag: BSU24220
Phenotypes of a mutant
- inactivation of spo0A restores beta-lactam resistance in a sigM mutant PubMed
- altered cell death pattern in colonies PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- activation of gene expression at the onset of sporulation (Spo0A regulon)
- coordination between DNA replication and initiation of sporulation by binding to sites close to the oriC (this limits re-initiation of replication) PubMed
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification: receives phosphorylation from Spo0B, dephosphorylation by Spo0E, direct phosphorylation by KinC upon potassium leakage PubMed
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure: 1QMP (with phosphorylated aspartate, Geobacillus stearothermophilus), 1FC3 (trans-activation domain, Geobacillus stearothermophilus), 1LQ1 (complex with DNA)
- UniProt: P06534
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: spo0A PubMed
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
- Tony Wilkinson, York University, U.K. homepage
- Imrich Barak, Slovak Academy of Science, Bratislava, Slovakia homepage
- Charles Moran, Emory University, NC, USA homepage
Your additional remarks
References
Reviews
The Spo0A regulon
Other original Publications