spoIVB

spoIVB
168

serine protease (site 1 protease), cleaves SpoIVFA to prepare it for CtpB cleavage, this results in pro-SigK processing/activation in the mother-cell, does also cleave CtpB

Locus
BSU_24230
Molecular weight
45.81 kDa
Isoelectric point
8.84
Protein length
426 aaSequenceBlast
Gene length
1281 bpSequenceBlast
Function
control of SigK activation
Product
serine protease
Essential
no
Synonyms
spoIVB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG5832 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,519,102 → 2,520,382
The protein
Catalyzed reaction/ biological activity
Self-cleaves 52-Val-|-Asn-53, 62-Ala-|-Phe-63 and 74-Val-|-Thr-75 at the N-terminus of SpoIVB (according to UniProt)
Protein family
PDZ protease PubMed
Domains
PDZ domain (aa 101 - 187) (according to UniProt)
Peptidase S55 domain (aa 188-426) (according to UniProt)
Structure
P17896 (AlphaFold)
Localization
secreted from the forespore to the intracellular space between the mother cell and the forespore PubMed
Expression and Regulation
Operons
Genes
spoIVB
Description
PubMed
Regulation
expressed during sporulation in the forespore (SigF, SigG, SpoVT) PubMed
Regulatory mechanism
SpoVT: activation, PubMed, in spoVT regulon
Sigma factors
SigF: sigma factor, PubMed, in sigF regulon
SigG: sigma factor, PubMed, in sigG regulon
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spoIVB

2025-03-09 07:05:37

ghost

66

acd97afd84625406d11a64dc87c9c0bf9c73ec31

16DC1EB96241822C6B6A8E05BC0AAAB06F7C0F9A

Biological materials
Mutant
BKE24230 (ΔspoIVB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCACTACTTCACTCTCC,  downstream forward: _UP4_TGACTGCCGGAGTTTCCGGC
BKK24230 (ΔspoIVB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCACTACTTCACTCTCC,  downstream forward: _UP4_TGACTGCCGGAGTTTCCGGC
Labs working on this gene/protein
Simon Cutting, London, UK homepage
References
Reviews
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Original Publications
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DBB3ED60A7D162B9F2830F81041BC661AA5EC1E7

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Time of last update: 2025-04-01 00:33:45

Author of last update: Jstuelk