BglC

• Description: endo-1,4-beta-glucanase
  
  

Gene name	bglC
Synonyms	eglS
Essential	no
Product	endo-1,4-beta-glucanase)
Function	beta-1,4-glucan degradation
Gene expression levels in SubtiExpress: bglC
MW, pI	55 kDa, 8.619
Gene length, protein length	1497 bp, 499 aa
Immediate neighbours	alsT, ynfE
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

The gene

Basic information

• Locus tag: BSU18130

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans (according to Swiss-Prot)

• Protein family: glycosyl hydrolase 5 (cellulase A) family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: extracellular (signal peptide) PubMed

Database entries

• Structure: 3PZT PubMed

• UniProt: P10475

• KEGG entry: [3]

• E.C. number: 3.2.1.4

Expression and regulation

• Operon: bglC (according to DBTBS)

• Expression browser: bglC PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1A751 (no resistance), PubMed, available at BGSC
  • 1A752 (no resistance), PubMed, available at BGSC
  • 1A976 (no resistance), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Yujuan Wang, Jun Wang
C-terminal flanking peptide stabilized the catalytic domain of a recombinant Bacillus subtilis endo-β-1, 4-glucanase.
Protein J: 2013, 32(4);246-52
[PubMed:23543074] [WorldCat.org] [DOI] (I p)

Camila R Santos, Joice H Paiva, Maurício L Sforça, Jorge L Neves, Rodrigo Z Navarro, Júnio Cota, Patrícia K Akao, Zaira B Hoffmam, Andréia N Meza, Juliana H Smetana, Maria L Nogueira, Igor Polikarpov, José Xavier-Neto, Fábio M Squina, Richard J Ward, Roberto Ruller, Ana C Zeri, Mário T Murakami
Dissecting structure-function-stability relationships of a thermostable GH5-CBM3 cellulase from Bacillus subtilis 168.
Biochem J: 2012, 441(1);95-104
[PubMed:21880019] [WorldCat.org] [DOI] (I p)

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

L M Robson, G H Chambliss
Endo-beta-1,4-glucanase gene of Bacillus subtilis DLG.
J Bacteriol: 1987, 169(5);2017-25
[PubMed:3106328] [WorldCat.org] [DOI] (P p)

R M MacKay, A Lo, G Willick, M Zuker, S Baird, M Dove, F Moranelli, V Seligy
Structure of a Bacillus subtilis endo-beta-1,4-glucanase gene.
Nucleic Acids Res: 1986, 14(22);9159-70
[PubMed:3024130] [WorldCat.org] [DOI] (P p)