RplN

• Description: ribosomal protein
  
  

Gene name	rplN
Synonyms
Essential	yes PubMed
Product	ribosomal protein L14
Function	translation
Gene expression levels in SubtiExpress: rplN
Interactions involving this protein in SubtInteract: RplN
MW, pI	13 kDa, 10.399
Gene length, protein length	366 bp, 122 aa
Immediate neighbours	rpsQ, rplX
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes, universally conserved proteins, phosphoproteins

This gene is a member of the following regulons

stringent response

The gene

Basic information

• Locus tag: BSU01260

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: ribosomal protein L14P family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-7 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • RplN-YqeL PubMed, the interaction serves to silence translation PubMed

• Localization:

Database entries

• Structure: 1WHI (Geobacillus stearothermophilus)

• UniProt: P12875

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: rpsJ-rplC-rplD-rplW-rplB-rpsS-rplV-rpsC-rplP-rpmC-rpsQ-rplN-rplX-rplE-rpsN-rpsH-rplF-rplR-rpsE-rpmD-rplO PubMed

• Expression browser: rplN PubMed

• Sigma factor:
  • SigA two promoters, about 140bp and 200bp upstream of the start codon PubMed

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed
  • strongly repressed in response to glucose starvation in M9 medium PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed