SacB
- Description: levansucrase
| Gene name | sacB |
| Synonyms | |
| Essential | no |
| Product | levansucrase |
| Function | utilization of sucrose, production of levan |
| Gene expression levels in SubtiExpress: sacB | |
| Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism, Stress | |
| MW, pI | 52 kDa, 6.175 |
| Gene length, protein length | 1419 bp, 473 aa |
| Immediate neighbours | pbpE, levB |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
utilization of specific carbon sources
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU34450
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Sucrose + (6)-beta-D-fructofuranosyl-(2->)(n) alpha-D-glucopyranoside = glucose + (6)-beta-D-fructofuranosyl-(2->)(n+1) alpha-D-glucopyranoside (according to Swiss-Prot)
- Protein family: glycosyl hydrolase 68 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- secreted (according to Swiss-Prot), extracellular (signal peptide) PubMed
Database entries
- UniProt: P05655
- KEGG entry: [3]
- E.C. number: 2.4.1.10
Additional information
Expression and regulation
- Regulatory mechanism:
- SacY: binding to a RNA switch (transcriptional antitermination) PubMed
- DegU-P: transcription activation PubMed
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Original publications
Clarita Olvera, Sara Centeno-Leija, Paulina Ruiz-Leyva, Agustín López-Munguía
Design of chimeric levansucrases with improved transglycosylation activity.
Appl Environ Microbiol: 2012, 78(6);1820-5
[PubMed:22247149]
[WorldCat.org]
[DOI]
(I p)
Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862]
[WorldCat.org]
[DOI]
(I p)
Kensuke Tsukahara, Mitsuo Ogura
Promoter selectivity of the Bacillus subtilis response regulator DegU, a positive regulator of the fla/che operon and sacB.
BMC Microbiol: 2008, 8;8
[PubMed:18197985]
[WorldCat.org]
[DOI]
(I e)
Y Pereira, M F Petit-Glatron, R Chambert
yveB, Encoding endolevanase LevB, is part of the sacB-yveB-yveA levansucrase tricistronic operon in Bacillus subtilis.
Microbiology (Reading): 2001, 147(Pt 12);3413-9
[PubMed:11739774]
[WorldCat.org]
[DOI]
(P p)
A M Crutz, M Steinmetz, S Aymerich, R Richter, D Le Coq
Induction of levansucrase in Bacillus subtilis: an antitermination mechanism negatively controlled by the phosphotransferase system.
J Bacteriol: 1990, 172(2);1043-50
[PubMed:2105292]
[WorldCat.org]
[DOI]
(P p)
S Aymerich, M Steinmetz
Cloning and preliminary characterization of the sacS locus from Bacillus subtilis which controls the regulation of the exoenzyme levansucrase.
Mol Gen Genet: 1987, 208(1-2);114-20
[PubMed:3039303]
[WorldCat.org]
[DOI]
(P p)
H Shimotsu, D J Henner
Modulation of Bacillus subtilis levansucrase gene expression by sucrose and regulation of the steady-state mRNA level by sacU and sacQ genes.
J Bacteriol: 1986, 168(1);380-8
[PubMed:2428811]
[WorldCat.org]
[DOI]
(P p)
P Gay, D Le Coq, M Steinmetz, E Ferrari, J A Hoch
Cloning structural gene sacB, which codes for exoenzyme levansucrase of Bacillus subtilis: expression of the gene in Escherichia coli.
J Bacteriol: 1983, 153(3);1424-31
[PubMed:6402497]
[WorldCat.org]
[DOI]
(P p)
