sacB

sacB
168

levansucrase

Locus
BSU_34450
Molecular weight
52.80 kDa
Isoelectric point
6.18
Protein length
473 aaSequenceBlast
Gene length
1422 bpSequenceBlast
Function
utilization of sucrose, production of levan
Product
levansucrase
Essential
no
E.C.
2.4.1.10
Synonyms
sacB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms

This gene is a member of the following regulons

Gene
Coordinates
3,536,012  3,537,433
Phenotypes of a mutant
defective in tomato root colonization in the presence of sucrose PubMed
The protein
Catalyzed reaction/ biological activity
[6)-β-D-fructofuranosyl-(2→](n) α-D-glucopyranoside + sucrose --> [6)-β-D-fructofuranosyl-(2→](n+1) α-D-glucopyranoside + D-glucose (according to UniProt)
Protein family
glycosyl hydrolase 68 family (single member, according to UniProt)
Structure
1PT2 (PDB) (complex with sucrose),  1OYG (PDB)
P05655 (AlphaFold)
Localization
secreted (according to Swiss-Prot),  extracellular (signal peptide) PubMed
Expression and Regulation
Operons
Genes
sacB-levB
Description
PubMed
Regulation
the RNA switch RNA is degraded by RNase Y PubMed
Regulatory mechanism
DegU: activation, DegU-P, PubMed, in degU regulon
SacY: antitermination, /antitermination via binding to a RNA switch, in sacY regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

sacB→levB

2025-03-28 11:15:26

Jstuelk

97

045fcc2eb89a1dc92a1a5a36944dc7ec173ff1cf

738CCB0FCDEF80A5E71672A01EA171EF191FFE25

Biological materials
Mutant
BKE34450 (sacB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCGTTCATGTCTCCTTTT,  downstream forward: _UP4_TAAAAACGCAAAAGAAAATG
BKK34450 (sacB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCGTTCATGTCTCCTTTT,  downstream forward: _UP4_TAAAAACGCAAAAGAAAATG
LacZ fusion
pGP564 (in pAC7), a series of RAT mutations in pAC7, all available in Jörg Stülke's lab
References
Reviews
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Original Publications
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AAA944BE7F01CE90F7730EECA16F3E4ED77D165A

Page visits: 24724

Time of last update: 2025-03-31 05:57:48

Author of last update: Jstuelk