RpsJ

• Description: ribosomal protein
  
  

Gene name	rpsJ
Synonyms	tetA
Essential	yes PubMed
Product	ribosomal protein S10 (BS13)
Function	translation
MW, pI	11 kDa, 10.207
Gene length, protein length	306 bp, 102 aa
Immediate neighbours	ybaC, rplC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

translation, essential genes, universally conserved proteins

This gene is a member of the following regulons

stringent response

The gene

Basic information

• Locus tag: BSU01150

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: ribosomal protein S10P family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P21471

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: rpsJ-rplC-rplD-rplW-rplB-rpsS-rplV-rpsC-rplP-rpmC-rpsQ-rplN-rplX-rplE-rpsN-rpsH-rplF-rplR-rpsE-rpmD-rplO PubMed

• Sigma factor:
  • SigA two promoters, about 140bp and 200bp upstream of the start codon PubMed

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:
  • pGP605 (in pAC6), available in Stülke lab
  • pGP1467 (in pAC7), available in Stülke lab

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Tom Coenye, Peter Vandamme
Organisation of the S10, spc and alpha ribosomal protein gene clusters in prokaryotic genomes.
FEMS Microbiol Lett: 2005, 242(1);117-26
[PubMed:15621428] [WorldCat.org] [DOI] (P p)