RrnO-16S

• Description: 16S rRNA
  
  

Gene name	rrnO-16S
Synonyms
Essential	no
Product	ribosomal RNA-16S
Function	translation
Gene length	1553 bp
Immediate neighbours	gyrA, trnO-Ile
Gene sequence (+200bp)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag:

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

• Structure:

Expression and regulation

• Operon: rrnO operon (rrnO-16S - trnO-Ile - trnO-Ala -rrnO-23S - rrnO-5S) PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • passive transcriptional regulation by cellular GTP concentration
  • downregulated by stringent response upon amino acid starvation PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Hideaki Nanamiya, Makiko Sato, Kenta Masuda, Mikiko Sato, Tetsuya Wada, Shota Suzuki, Yousuke Natori, Masato Katano, Genki Akanuma, Fujio Kawamura
Bacillus subtilis mutants harbouring a single copy of the rRNA operon exhibit severe defects in growth and sporulation.
Microbiology (Reading): 2010, 156(Pt 10);2944-2952
[PubMed:20634236] [WorldCat.org] [DOI] (I p)

Yousuke Natori, Kazumi Tagami, Kana Murakami, Sawako Yoshida, Osamu Tanigawa, Yoonsuh Moh, Kenta Masuda, Tetsuya Wada, Shota Suzuki, Hideaki Nanamiya, Yuzuru Tozawa, Fujio Kawamura
Transcription activity of individual rrn operons in Bacillus subtilis mutants deficient in (p)ppGpp synthetase genes, relA, yjbM, and ywaC.
J Bacteriol: 2009, 191(14);4555-61
[PubMed:19447912] [WorldCat.org] [DOI] (I p)

Libor Krásný, Richard L Gourse
An alternative strategy for bacterial ribosome synthesis: Bacillus subtilis rRNA transcription regulation.
EMBO J: 2004, 23(22);4473-83
[PubMed:15496987] [WorldCat.org] [DOI] (P p)

N Ogasawara, S Moriya, H Yoshikawa
Structure and organization of rRNA operons in the region of the replication origin of the Bacillus subtilis chromosome.
Nucleic Acids Res: 1983, 11(18);6301-18
[PubMed:6312418] [WorldCat.org] [DOI] (P p)

Additional publications: PubMed