Icd

• Description: isocitrate dehydrogenase
  
  

Gene name	icd
Synonyms	citC
Essential	no
Product	isocitrate dehydrogenase
Function	TCA cycle
MW, pI	46 kDa, 4.833
Gene length, protein length	1269 bp, 423 aa
Immediate neighbours	citZ, mdh
Gene sequence (+200bp)	Protein sequence
Genetic context

The gene

Basic information

• Coordinates:

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated PubMed, PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: attached to the membrane PubMed

Database entries

• Structure: 1HQS

• Swiss prot entry:

• KEGG entry:

• E.C. number: 1.1.1.42

Expression and regulation

• Operon:
  • citZ-icd-mdh
  • icd-mdh

• Sigma factor: SigA

• Regulation: citZ: catabolite repression (CcpA), repression by glucose + glutamate (CcpC)

   icd: constitutive 

• Regulatory mechanism: CcpA: transcription repression, CcpC: transcription repression

• Additional information:

Biological materials

• Mutant: GP666 (spc), GP672 (erm), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

1. Matsuno K, Blais T, Serio AW, Conway T, Henkin TM, Sonenshein AL (1999) Metabolic imbalance and sporulation in an isocitrate dehydrogenase mutant of Bacillus subtilis. J Bacteriol 181:3382-3391. PubMed
2. Singh SK, Miller SP, Dean A, Banaszak LJ, LaPorte DC (2002) Bacillus subtilis isocitrate dehydrogenase. A substrate analogue for Escherichia coli isocitrate dehydrogenase kinase/ phosphatase. J Biol Chem 277: 7567-7573. PubMed
3. Hahne et al. (2008) From complementarity to comprehensiveness - targeting the membrane proteome of growing Bacillus subtilis by divergent approaches. Proteomics 8: 4123-4136 PubMed
4. Eymann et al. (2007) Dynamics of protein phosphorylation on Ser/Thr/Tyr in Bacillus subtilis. Proteomics 7: 3509-3526. PubMed
5. Levine et al. (2006) Analysis of the dynamic Bacillus subtilis Ser/Thr/Tyr phosphoproteome implicated in a wide variety of cellular processes. Proteomics 6, 2157-2173. PubMed
6. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed