ccpC
168
transcriptional repressor of citZ and citB
Locus
BSU_14140
Molecular weight
33.76 kDa
Isoelectric point
5.93
Function
regulation of tricarboxylic acid branch of the TCA cycle
Product
transcriptional repressor (LysR family)
Essential
no
Synonyms
ccpC, ykuM
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0583 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,486,045 → 1,486,926
The protein
Protein family
LysR family (according to UniProt)
HTH lysR-type domain (aa 1-58) (according to UniProt)
Structure
5Z72 (PDB) (from B. amyloliquefaciens, 96% identity)
Expression and Regulation
Operons
Genes
Description
Regulation
Regulatory mechanism
Sigma factors
SigA: sigma factor, in sigA regulon
Biological materials
Mutant
GP3593 (ΔccpC::phleo), available in Jörg Stülke's lab
MGNA-A774 (ykuM::erm), available at the NBRP B. subtilis, Japan
GP1442 (aphA3) , available in Jörg Stülke's lab
BKE14140 (ΔccpC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTCGTCCTCCTTTTTGT, downstream forward: _UP4_TAAAGACAGCGAGGTGCTGT
BKK14140 (ΔccpC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTCGTCCTCCTTTTTGT, downstream forward: _UP4_TAAAGACAGCGAGGTGCTGT
GP3593 (Δ''ccpC::phleo''), available in Jörg Stülke's lab
Expression vectors
for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP706, available in Jörg Stülke's lab
pGP3929 (C-terminal Strep-tag, purification from E. coli, in pGP574), available in Jörg Stülke's lab
pGP3936 (N-terminal Strep-tag, purification from E. coli, in pGP172), available in Jörg Stülke's lab
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
LacZ fusion
Labs working on this gene/protein
Linc Sonenshein, Tufts University, Boston, MA, USA Homepage
References
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Time of last update: 2025-04-02 02:19:40
Author of last update: Dennis.wicke