NfrA

• Description: Spx-dependent FMN-containing NADPH-linked nitro/flavin reductase, stress protein
  
  

Gene name	nfrA
Synonyms	ywcG, ipa-43d
Essential	no
Product	FMN-containing NADPH-linked nitro/flavin reductase
Function	unknown
Gene expression levels in SubtiExpress: nfrA
Metabolic function and regulation of this protein in SubtiPathways: nfrA
MW, pI	28 kDa, 5.732
Gene length, protein length	747 bp, 249 aa
Immediate neighbours	ywcH, rodA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

electron transport/ other, resistance against oxidative and electrophile stress

This gene is a member of the following regulons

SigD regulon, Spo0A regulon, Spx regulon

The gene

Basic information

• Locus tag: BSU38110

Database entries

• BsubCyc: BSU38110

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: possesses an NADH oxidase activity that leads to high concentrations of oxygen peroxide and an NAD(+) degrading activity leading to free nicotinamide PubMed

• Protein family: flavin oxidoreductase frp family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU38110

• Structure: 3N2S PubMed

• UniProt: P39605

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: nfrA-ywcH PubMed

• Expression browser: nfrA PubMed

• Sigma factor: SigA PubMed, SigD PubMed

• Regulation:
  • repressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed
  • Spx: transcription activation PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 222 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 616 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 560 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 960 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Sylvie Cortial, Philippe Chaignon, Bogdan I Iorga, Stéphane Aymerich, Gilles Truan, Virginie Gueguen-Chaignon, Philippe Meyer, Solange Moréra, Jamal Ouazzani
NADH oxidase activity of Bacillus subtilis nitroreductase NfrA1: insight into its biological role.
FEBS Lett: 2010, 584(18);3916-22
[PubMed:20727352] [WorldCat.org] [DOI] (I p)

Le Thi Tam, Haike Antelmann, Christine Eymann, Dirk Albrecht, Jörg Bernhardt, Michael Hecker
Proteome signatures for stress and starvation in Bacillus subtilis as revealed by a 2-D gel image color coding approach.
Proteomics: 2006, 6(16);4565-85
[PubMed:16847875] [WorldCat.org] [DOI] (P p)

Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647] [WorldCat.org] [DOI] (P p)

Shunji Nakano, Michiko M Nakano, Ying Zhang, Montira Leelakriangsak, Peter Zuber
A regulatory protein that interferes with activator-stimulated transcription in bacteria.
Proc Natl Acad Sci U S A: 2003, 100(7);4233-8
[PubMed:12642660] [WorldCat.org] [DOI] (P p)

C Moch, O Schrögel, R Allmansberger
Transcription of the nfrA-ywcH operon from Bacillus subtilis is specifically induced in response to heat.
J Bacteriol: 2000, 182(16);4384-93
[PubMed:10913069] [WorldCat.org] [DOI] (P p)

S Zenno, T Kobori, M Tanokura, K Saigo
Purification and characterization of NfrA1, a Bacillus subtilis nitro/flavin reductase capable of interacting with the bacterial luciferase.
Biosci Biotechnol Biochem: 1998, 62(10);1978-87
[PubMed:9836433] [WorldCat.org] [DOI] (P p)

C Moch, O Schrögel, R Allmansberger
The sigmaD-dependent transcription of the ywcG gene from Bacillus subtilis is dependent on an excess of glucose and glutamate.
Mol Microbiol: 1998, 27(5);889-98
[PubMed:9535080] [WorldCat.org] [DOI] (P p)