OpuBD

• Description: choline ABC transporter (membane protein)
  
  

Gene name	opuBD
Synonyms	proZ
Essential	no
Product	choline ABC transporter (membane protein)
Function	compatible solute transport
Gene expression levels in SubtiExpress: opuBD
Interactions involving this protein in SubtInteract: OpuBD
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	23 kDa, 10.06
Gene length, protein length	678 bp, 226 aa
Immediate neighbours	yvaQ, opuBC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

ABC transporters, coping with hyper-osmotic stress, membrane proteins

The gene

Basic information

• Locus tag: BSU33700

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: CysTW subfamily (according to Swiss-Prot)

• Paralogous protein(s): OpuCD

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • OpuBA-(OpuBB-OpuBD)-OpuBC PubMed

• Localization: cell membrane PubMed

Database entries

• Structure:

• UniProt: P39775

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: opuBA-opuBB-opuBC-opuBD PubMed

• Expression browser: opuBD PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by salt stress PubMed
  • induced by choline (GbsR) PubMed

• Regulatory mechanism:
  • GbsR: transcription repression PubMed

• Additional information:
  • An antisense RNA is predicted foropuBD PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Tamara Hoffmann, Erhard Bremer
Protection of Bacillus subtilis against cold stress via compatible-solute acquisition.
J Bacteriol: 2011, 193(7);1552-62
[PubMed:21296969] [WorldCat.org] [DOI] (I p)

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

R M Kappes, B Kempf, S Kneip, J Boch, J Gade, J Meier-Wagner, E Bremer
Two evolutionarily closely related ABC transporters mediate the uptake of choline for synthesis of the osmoprotectant glycine betaine in Bacillus subtilis.
Mol Microbiol: 1999, 32(1);203-16
[PubMed:10216873] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)

G Nau-Wagner, J Boch, Le Good JA, E Bremer
High-affinity transport of choline-O-sulfate and its use as a compatible solute in Bacillus subtilis.
Appl Environ Microbiol: 1999, 65(2);560-8
[PubMed:9925583] [WorldCat.org] [DOI] (P p)